*Prices are based on a spiral-bound dissertation of 40 pages , printed single-sided , in black , on 80-90gsm paper, including a clear outer front and back cover .
**Prices are based on a hardback-bound dissertation of 200 pages , printed single-sided , in black , on 90-100gsm paper.
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Book1one will give your thesis or dissertation the professional look it deserves..
If you are looking for a world class company to print and bind your thesis or dissertation, you will find that Book1One will give your document the high quality attention it requires and deserves. Graduate students from all over the country have used our services, and we are listed at many colleges and universities as a reliable resource for hardcover and softcover thesis and dissertation book printing.
We understand that theses and dissertations comprise a special category in the book publishing world. They represent the culmination of a large body of work and achievement at a very high academic level – and are recognized symbols of professional accomplishment.
Cloth and Faux Leather Color Choices:
NOTE: Colors may not display accurately due to differences in monitor settings
Why choose Book1One to print your thesis or dissertation?
If your foil stamping involves basic text and numbers, such as book title, author’s name, school and date, Book1One can assemble your cover for you at no additional cost to use. If you wish us to provide this service to you, when you place your order, upload a PDF file indicating what you would like printed on your front cover and spine and approximate positioning. We will fit and center the type using the Time New Roman font. For more on foil stamping click here .
Book1One is one of very few book manufacturers who has a website price calculator that will enable you to obtain an accurate price quote without having to place a phone call or submit an email. It’s fast, easy, and our customers love it!
Simply insert the specifications of your book project and, with one click, see what the price of your project will be.
If you have completed the design and layout of your thesis or dissertation and are ready to upload your print ready PDF file(s) to us and place your order, simply click on the red “Place Order” button located at the top of your screen. You will then be taken through the simple three step process of (1) creating your personal account, (2) uploading your cover and page files and (3) completing the checkout procedure.
Our guarantee to you is that we will give your project our utmost personal attention to ensure that, when finished, your books meet the highest quality production standards.
I just received my book order of an early 1900's scrapbook replica you printed for me - and it is perfect! I can't wait to show it to my client and display it at a networking show next month. Many thanks for a great job.
Wendy McSweeney Powhatan, VA
It has been a pleasure working with you on my photo book project. Many of my colleagues have complimented the book's photo colors and quality.
R. C. Livesay Author & Photographer of The Secret Life of the Grand Lake Pelican
You did a wonderful job with our book project. Everything was done exactly the way we had envisioned it.
Fairlee MacEslin Woodstock, VT
We received our books today, and they look fabulous! You do quite a professional job. We will certainly keep you in mind for any future projects.
Shelley White Uriccio, Howe, Krell, Jacobson,Toporek, Theos & Keith Charleston, SC
Wow! You all did an amazing job printing our proposal books. Thank you so much for your most efficient and beautiful work. We'll definitely be doing this again.
Dlorah deVore CDI Contractors, LLC Little Rock, AR
My book looks amazing! This is the first time I have used your services, and I could not be happier. Thank you for exceeding my expectations in every way.
Dan M. Chicago, IL
I received my cookbooks today and wanted you to know how much I appreciate the wonderful printing and binding job you did. You have helped put together a 'treasured keepsake' for our family.
Shan Wilkins Berryville, VA
I received my books today, and all I can say is OMG! They are so much more than I expected. Thank you for providing such a wonderful service and product.
Jim D. Corydon, IN
Book1One has been an amazing resource for launching my publishing business. I couldn't have done it without their timely service and high-quality results. Thanks for helping memorylanejane.com publish some amazing Life Story Books!
Lauren Befus Founder, Memory Lane Jane
I am a designer who has ordered several low-volume books for my clients from Book1One over the last few years and have been extremely pleased with the quality as well as the customer service. I have created both hard cover and soft cover books, and the website has useful templates for formatting the book layout so everything comes together seamlessly. I also love the instant quote generator on the website, it helps to see how the different options impact the pricing without having to go back and forth with an estimator or sales rep. I will definitely keep using Book1One!
Kathryn D'Amanda Graphic Designer, Mill Race Design
Call (716) 883-5185
Do you need your thesis bound, thesis & dissertation binding.
Trust your thesis binding to QBS, a company that reveres education, and the work you have achieved! Our pricing is reasonable. We know you’ve just spent a bundle on your education! We have been helping graduate students preserve their thesis work for years. It’s simple. Whether you want a soft cover or a hard cover -- both are available. We follow the instructions of the school you attend. We use all types of materials, including cloth, paper and leather. If your school no longer requires you to print and bind your dissertation . . . have it bound beautifully for yourself!
Download our Thesis Binding Information Flyer.
Hard Cover Value Pricing Our value pricing for hard cover thesis binding is $55 per book.
Includes hard cover, we use imitation leather
Choose from many colors, including black, navy, medium blue, brown, green and burgundy
With gold or silver foil stamping on spine and front cover
Soft Cover Value Pricing Our value pricing for soft cover thesis binding is $40 per book.
Includes soft cover, we supply the cover stock
Choose black or white cover
Printing Services
QBS also provides printing services on a variety of paper stocks. The prices are as follows:
25% cotton with watermark: $.45 b/w $.60 for color
24# bond copy paper: $.30 b/w $.40 color
100% cotton with watermark: $.55 b/w $.75 for color
Turn around is usually 3 weeks.
If you need your theses sooner, rush charges may apply depending on our schedule:
5 to 10 days: an additional $20 per book
4 days or less: an additional $50 per book
"My bound dissertation books arrived today, and you did an amazing job. It looks excellent. I am so thrilled and happy. I will definitely use your services again (going back to school) and I have forwarded information to my other college cohorts. Thank you so much." B.
Step 1: Fill Out the Order Form Download our PDF Thesis Order Form Important: If you have difficulty filling out our order form via the PDF, please print the PDF form and fill it out. Scan and email to [email protected] or fax it to (716) 883-1598. Step 2: Upload Your Files Upload your files to our secure WeTransfer FTP (file transfer protocol) site.
https://qualitybindery.wetransfer.com/ Step 3: We Email Your Price Confirmation Step 4: PayPal Payment
Send payment directly from your PayPal account using our PayPal email address: [email protected] Step 5: Delivery Options
Click here for hours and directions
Quality Bindery Services Inc.
501 Amherst St. Buffalo, NY 14027
We ship per your instructions on your order form.
For more information call (888) 883-1266 toll-free or (716) 883-5185. We are here to congratulate you and to help you with this next exciting step in your career!
Here is an example of one of Quality Bindery Services' great Buffalo, New York customers -- D'Youville College.
D'Youville graduates, c lick the below image to download your college's order form. Others use the form in Step 1.
We think all of the work you completed to get your doctorate degree deserves a place on your bookshelf. It serves as part of your life legacy and can be handed down to your family's future generations.
BOUND THESIS TODAY!
A quick, easy and cost-effective way to have your thesis or dissertation professionally printed and bound., the 1 day option is only available monday to thursday, any 1 day orders placed on a friday will not be dispatched until monday, orders for the one day option must be placed by 10am for next day delivery..
Helix Binders are a major provider of dissertation / thesis print & bind for students throughout the UK.
With the ability to match all UK university specifications you can trust Helix Binders to produce your work in the highest quality.
First impressions count.
Everyone knows this little phrase and it applies completely to submitting your thesis too. You’ve poured man-hours into researching, writing and proofing your Thesis – the last thing you want is to make a poor impression by submitting a substandard printed document.
A well printed and bound document will reflect all the hard work that has gone into creating the content within it.
We’ve been binding dissertations and thesis for many years and know a thing or two about it. From the feedback we’re received, a beautifully bound dissertation has much more impact. Your publication is in good hands.
You’ll want the overall finish to be perfect. Neatly trimmed, durable, and free of blemishes. We inspect each document before we ship it out, giving you peace of mind that it has been printed with care and attention to detail.
Many universities have their own thesis binding specifications for submitted documents and we can bind to those requirements. This ensures that you not only comply but also make the best possible impression.
Using the latest digital printing technology, you can be sure that the print quality will be excellent and we don’t skimp on the paper either. Our standard weight is 100gsm, making sure the print is legible and the document is a pleasure to handle too.
Our thesis / dissertation binding service offers you a choice of covers and finishes, meaning it is customised to you.
We also offer a variety of extra options allowing you to add even more uniqueness to your document and make it stand out.
You have a variety of delivery options too, including Next-day delivery with a 1 hour window. You won’t have to hang around all day waiting for that important thesis to arrive. We know that there is no point in getting a thesis printed and bound to a professional standard, only to find that it has been damaged in the post. We take care in packing and delivering your thesis so that it arrives in the pristine condition you’d expect.
Having choice means you have a say in the final cost. Our printing and binding processes are streamlined to ensure low waste and a fast turn-around – keeping costs to a minimum and giving you the best possible finish at a great price. We have a quick cost calculator to help you get an idea of the choice and the pricing before you even place an order.
Having survived the stress of compiling your thesis , you’ll want the submission process to be smooth and easy.
We have a wide range of applications and will process your files in most common formats. If you are unsure of what format to submit your document, please do not hesitate to contact us [email protected] .
We have produced thesis binding to students from all over the country, here’s a quick look at just how much of the UK we cover:
It doesn’t matter which university you attend, or where you are in the UK – we can print it, bind it and ship it for you.
Graphic from studyin-uk.com
Hi. Ordered my wife's PhD Thesis to be bound, all 336 pages, last week. It's arrived! We are so pleased with the quality and service. Thank you. Stephen, Lynn and Mabel the dog. One happy house.
Thank you very much for the binding. I have received the thesis and it is very well done.
Kind regards, Kevin
I binded my dissertation in 2021, and I am totally satisfied with the result! It is beautifully binded and very proud of the book!
What fantastic service, and what a great finished product. Iain was brilliant in ironing out a few glitches (caused by me tbh). I can't praise him or the service provided highly enough. If you want your thesis bound professionally you've found the right place.
Just received my bound dissertation from Helix. I had to write this brief message to say thank you so much, it is absolutely stunning. The service, quality, and simplicity of the process is amazing. I cannot recommend your company enough, I will definitely be using you for future binding projects. Many thanks, Dave.
Find out more.
For information about any of our services, please feel free to call 01324 629025 or email us .
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One of the best things is the feeling you get when you’ve finished writing your thesis. After months of research and writing, at long last, your paper, thesis, or dissertation is finally done. What’s left to do is the printing and binding of your thesis. But still, you need to find out the best printing and binding services and choose a style that makes you proud of the final result of your work.
There are a few things to consider when choosing how to print and bind your thesis or dissertation: the quality of the paper, the material, and color of the binding. A few other details can be customized, such as the ribbons and corner protectors. To help you decide, we have selected a few options from our partner, BachelorPrint.
BachelorPrint is your go-to expert when it comes to printing and binding your thesis. The online market leader offers a wide range of bindings and will transform your thesis into a work of art.
The ultimate : with their free express shipping they guarantee that your thesis will be in your hands within a few days!
You’ve made it! You’ve finished writing your thesis. Now all that’s left is printing and binding and knocking your examiner’s socks off! That’s why we recommend leather bookbinding .
A leather book has the best quality for printing and binding your thesis . Your thesis will look like a classy book.
Choosing this type of binding will make your thesis stand out from the rest and leave a lasting impression on your examiner. Combine that with additional options such as customized embossing, corner protectors, and a ribbon bookmark, and after that process your thesis you’ll have a one-of-a-kind, sophisticated-looking book.
There are two options available with this leather book: Premium leather book and standard leather book. Whereas the premium leather book has a matte finish, a standard leather book has a glossy finish. However, both are done in leather-look and consist of a solid and sturdy cover.
Which leather book you choose for your thesis binding is a matter of personal preference.
Deciding on a leather book when printing and binding your thesis gives you the option to upgrade it to your tastes and create a one-of-a-kind binding. Here are the upgrade options:
Corners protectors:
Adds a touch of class
Prevents the corners from binding
Available in silver, black & gold
Ribbon Bookmark:
Enhance your book’s appearance
Can be used as a bookmark (your examiner will be grateful!)
Available in white, black, blue, silver & gold
Customized embossing:
E.g.: University logo and title of your dissertation on the cover
Embossed spine optional
Available in black, silver & gold
You can use BachelorPrint preview tool see how your dream binding would look like:
Of all the different types of binding, thermal binding is a classic. One of the features is the transparent front cover: it shows off the title page of your dissertation as well as the title of your thesis. This means that attention is immediately drawn to the topic of your dissertation.
Looking to make an impact with your thesis? In that case, the softcover is just for you! You have free reign and can decide what you want your cover to look like: Logos, colors, photos, or different fonts – you’re the one who decides what goes on the cover of your softcover. That is why this type is ideal for more creative fields of study.
Note: Designing a one-of-a-kind binding that looks super original is tempting, but remember to keep it aligned with your field of studies and professional looking.
Of all the different types of binding, spiral binding is the sleekest and simplest type BachelorPrint has to offer. It has a transparent front cover. This allows the examiner to see the topic of your Master’s thesis or dissertation right away. However, spiral binding is better for printing shorter dissertations.
Some professors will require that you use spiral binding for printing your thesis. If your post-secondary institution has no guidelines regarding printing and binding, then you should make sure that the “packaging” of your thesis matches the contents: If you are looking into printing and binding your Master’s thesis, we definitely recommend the leather book over spiral binding.
Leather book | Thermal binding | Softcover | Spiral binding | |
---|---|---|---|---|
Page limit: | 10 – 370 pages | 2 – 450 pages | 20 – 150 pages | 1 – 300 pages |
Colors | Blue, black, red, green | Blue, red, green, gray, black, yellow, white | Customizable | Blue, red, green, gray, black, yellow, white |
Upgrades | – Corner protectors – Customized embossing – Ribbon bookmark | – | – Customized cover design | – |
Appearance | Matte or slightly glossy finish, sturdy, leather-look cardboard cover | Textured cardboard back cover, clear front cover | Satin matte, printable cover | Textured cardboard back cover, clear front cover |
Price | From €15.90 | From €9.90 | From €11.90 | From €4.90 |
It’s one thing to know what thesis binding to choose when printing your thesis. But there are lots of helpful and important tips that you should consider when it comes to printing. We sat down with BachelorPrint to put together some exclusive advice just for you:
Tip #1- Choosing the right paper: Most printing services use 80 g/m² paper by default. However, 80 g/m² paper is relatively thin and as a result, the print on the reverse side will shine through if you are printing double-sided.
Tip: Remember that printing and binding your thesis is not something you do every day – be sure to use high-quality paper and select 100 g/m² paper – if printing double-sided, we actually recommend 120 g/m² paper. BachelorPrint automatically uses 100 g/m² paper.
Tip #2- Printing single-sided or double-sided: It’s totally up to you whether you print your thesis single-sided or double-sided. Make sure you check the examiner’s guidelines first – if there are none, the choice is yours!
Tip: When printing double-sided, make sure your page numbers are formatted correctly. Uneven page numbers should always be on the right-hand page, and even page numbers should be on the left-hand page.
Tip #3- Printing in color: It’s up to you whether you print in color or black and white. This depends on your thesis. If your dissertation has lots of charts and photos, color printing would look better.
Tip: Color printing gives your thesis a classier appearance. But take note! Too much color and your thesis will look less professional.
Tip #4- Number of copies: You can assume that two members of the examination board will read your thesis. Furthermore, the person or organization you did your internship with might also like a copy of your thesis. Last but not least, you would probably also like to own a copy of your dissertation, Master’s thesis, or research paper .
Tip: Find out beforehand, how many copies you’ll need. We generally recommend making 4 to 5 copies. Of course, the type of binding is up to you.
Tip #5- Cost: The cost of printing and binding your thesis is based on various factors: Paperweight, color printing, and actual printing. Many service providers often add a surcharge when you opt for color printing or thicker paper.
Tip: BachelorPrint printing expert automatically uses 100 g/m² paper, and does not even add a surcharge. The same applies for color printing: whether you choose black/white or color, BachelorPrint charges the same.
Do you need to transcribe interviews for your thesis?
Ready to print?
Upload your files now!
You can now order your thesis printing & binding online with our new site: printservices.mit.edu
You put a great deal of work into your thesis. Now we can help you package your efforts with professional polish and style. From choosing the right paper stock to cover options and binding, we will walk you through the MIT thesis specifications to present your work with the high-quality look and feel it deserves.
MIT Copytech uses MIT-required thesis paper for all thesis print orders. Approved thesis paper is also available for purchase by the ream (500 sheets). We have an Athena thesis printer in our 11-004 location.
We provide hard-cover bookbinding for thesis printing. You can choose from an array of color choices as well as gold lettering print options on the cover and spine. Economical soft-cover binding choices are also available. Thesis covers and binding clips (required for thesis submission) can also be obtained through MIT Copytech.
$.06 | |
$30.00 (500 sheets) |
| |
$90.00 | |
$78.00 |
(Please note that the binding colors on screen may appear slightly different than in person.)
$2.25 each | |
$2.50 each | |
$2.50 each |
$2.00/set | |
$.10 | |
| $.25 |
Your thesis is the culmination of your higher education journey. The Multimedia and Print Center will help you recreate and preserve your important work with professional copying and binding services.
Place your order using our online form .
Pricing is subject to change. Exact charges will be calculated at the time of order.
Customers must provide two blank sheets of paper for each thesis as front and back end sheets. If you submit your thesis electronically, please provide it as a PDF. All emailed PDF files are final. Be aware that we cannot be held responsible for any variations between the electronic file you sent us and your thesis’s final printed version
We offer binding of periodicals for Penn State-related materials. Please contact us for available options and pricing.
Custom Penn State Binding: $42.00
· Penn State mark stamped on the front cover in gold or white · Buckram cover in black or blue · Gold/white lettering on the spine and front cover · Double-fan adhesive binding up to 2” thick-over 2” is an additional $4.25 per
Standard Binding: $35.00
· No Penn State mark stamped on the front cover · Buckram cover in black · Gold lettering on the spine and front cover · Double-fan adhesive binding up to 2” thick-over 2” is an additional $4.25 per
Coil Binding: $7
· Clear transparency on the front · Black or blue vinyl backing · Black coil
Tape (Thermal) Binding: $7
· Up to 325 sheets · Black or blue tape
Black and White 10 cents per sheet
• 25% cotton watermark paper • 24# laser paper
Color Print 60 cents per sheet
Cutting and pasting should be minimized for optimal copy quality. All fold-out, landscape, and color pages should be noted on the form.
Supplied copies are the responsibility of the customer and will be bound as submitted.
Pockets can be created for special enclosures for materials less than 1/8” thick at an additional $10 per book
To ensure timely delivery of your books, payment must be made in full at the time the order is placed. We accept Apple Pay, Lion Cash, Discover, American Express, Visa, and MasterCard.
Please note that all personal sales are subject to 6% PA sales tax.
Orders take approximately four weeks.
If you are supplying all the copies you need:
• You will be contacted when your order is available for pickup. • The finished copies will be available for pick-up at The Multimedia and Print Center, located in the Hostetter Business Services Building.
We also offer shipping of your bound volumes by Courier to on-campus locations or FedEx. Shipping charges will be calculated according to weight, region, and class. Charges must be paid at the time the order is placed.
** Please Note: FedEx does not deliver to P.O. boxes**
Contact information:.
The Multimedia and Print Center 101 Hostetter Business Services Building Services Road (15-minute curbside parking available) Located across from the Arboretum Phone: 814-865-7544 Hours of Operation: Monday-Friday, 8:00 am-4:30 pm
With real-time updates, you can manage your availability with precision. Customers can see open slots and make bookings without the risk of double-booking or scheduling conflicts.
Our user-friendly interface ensures that both your staff and customers can make, modify, or cancel bookings effortlessly. The intuitive design reduces the learning curve, allowing everyone to get on board quickly.
Set your booking policies, such as cancellation terms, minimum booking times, and required deposits, to suit your business needs. Customize the system to match your brand and operational requirements.
2. real-time availability:, our service.
Why choose us, why choose us.
Our Booking Binding Service is tailored to meet the unique needs of your business. We understand that efficient scheduling is crucial to your success, and we're committed to providing a reliable and feature-rich solution. Join the many businesses that trust us to handle their bookings with professionalism and ease.
Get started today and experience the difference our Booking Binding Service can make for your business. Contact us for a demo or sign up now to take control of your reservations like never before!
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Saket Nagar Colony, Lanka, Varanasi, Uttar Pradesh 221005
+91 97952 55995
Thesis binding is a specialised business so you should be wary of ‘bookbinders’ who have no experience binding theses. It is worth shopping around as costs can vary significantly. Some companies offer an online service.
It is also a good idea to go to the library and check the inside covers of theses, to see if they contain the name of the binder. That way you get to see the quality of the finished product as well.
Please note that PhD and MPhil must adhere to GRS guidelines for binding (scroll down to section 7.1.9). Requirements for binding of Masters by Research theses can vary across faculties, so check with your administrative officer for details.
Below is a list of thesis binders in Melbourne and Sydney. This is by no means a complete list of thesis binders, feel free to “google” others – we make no recommendation for any particular bindery.
For DE graduate students or for those who can’t get to a bindery in person, the whole process can usually be managed by email – check with your preferred binder.
Doncaster bookbinding.
www.doncasterbookbinders.com.au Factory 1/33 Ebden St Moorabbin 3189 (03) 9555 4633 Note: Pick-up and delivery service to the GRS office on Mondays.
whiteslaw.com.au/thesis_index 802- 804 Glenhuntly Rd Caulfield South, 3162 (03) 9523 6026
www.retail.monash.edu.au/printservices/ Phone on 9903 2135 or [email protected] Note: It can be arranged for bound copies to be delivered to GRS.
Pickup and delivery service 0419 577 094 [email protected]
Robel Bookbinders of Distinction 17 Sugarloaf Rd, Beaconsfield Upper VIC 3808 (03) 5944 4977 [email protected]
Allbook bindery.
www.allbookbindery.com.au 91 Ryedale Road West Ryde, NSW 2114 (02) 9807 6026
www.worldofprint.com.au Shop 3, 702 Harris Street Ultimo / Broadway (02) 9212 4144
Got a question or request about anything at all? Get in contact with one of our staff members – we’d love to hear from you. Alternatively, drop by the MGA office at Clayton or Caulfield so we can help!
Curious to know who’s representing you at the moment? Get to know your elected representatives , what they do and how you can get involved.
The MGA employs Monash graduate students to fill the receptionist roles at the Clayton and Caulfield offices. When a vacancy arises we advertise the position through our email newsletter and on our website .
FM Printing Mehal aims to assist everyone by offering convenient Printing & Binding services.
Our focus is on supporting females who face challenges going outside, as well as individuals in other cities dealing with high market rates. Customers can easily access our services via call or whatsapp for hassle-free experience.
Have a fantastic day.
0322-3714488 , 0331-4898223
We are specialist for Thesis Binding for all over the world.
We are providing all kind of content writing e.g. Assignment Writing & Thesis Writing.
All kind of printing facilities available.
What customer say
As the founder of this business, I am committed to providing high-quality services to all of our customers. We offer a range of printing services, e.g. thesis / assignment writing, formatting, proofreading, thesis binding, visiting cards, pamphlet, brochure, mug / shirt print and much more. Whatever your printing needs may be, we are here to help. Thank you for choosing us, and we look forward to serving you soon!
I am expert for thesis formatting, designer and consultant.
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until file(s) become available
Embryonic development relies on the complex interplay of epigenetic regulation, timely expression of genes, signal transduction pathways, and diverse morphological changes. The heart is the first organ to form during mammalian embryonic development. The proper development of the heart is critical to supply nutrients and oxygen to other cell types of the organism. Most cells that comprise the heart originate from the mesoderm post-gastrulation. Cardiomyocytes are the predominant cell type and confer function to the heart via contractile activity. The development and proliferation of cardiomyocytes ceases shortly after birth, where cardiomyocytes only nucleate and increase in size. Consequently, cardiomyocyte insufficiency underlies most cardiovascular diseases, a leading cause of death globally.
Vascular endothelial zinc finger 1 (VEZF1) is a transcription factor expressed predominantly in mesoderm during development. Previous studies from our lab show that the loss of VEZF1 impairs the differentiation of embryonic stem cells into endothelial cells, a cell type derived from mesoderm. Other published studies also show that Vezf1 loss impairs cardiomyocyte growth in Zebrafish and hematopoietic cell differentiation. Our work here describes a detailed investigation of the role of Vezf1 in the differentiation of mesoderm and cardiomyocytes using mouse embryonic stem cell (ESC) differentiation as a mammalian model system. We initially developed an efficient method, known as the Wnt Switch method, to differentiate ESCs into cardiomyocytes. Our technique relies on the treatment of differentiating ESCs with small molecule inhibitors: i) CHIR99021, which induces mesoderm development via the activation of Wnt signaling in the first 48 hours of differentiation, followed by ii) XAV939, which inhibits Wnt signaling and drives mesoderm cells toward cardiomyocyte differentiation pathway. The Wnt Switch method significantly increases the efficiency of cardiomyocyte derivation (86%) from ESC compared to published methods (56%).
Interestingly, the Wnt Switch method showed that despite the external stimulation of Wnt signaling, Vezf1 KO cells are unable to differentiate into cardiomyocytes and show reduced expression of mesodermal genes 48 hrs post-differentiation. To better understand the stage-specific role of Vezf1 in cardiomyocyte development, we generated doxycycline-inducible Vezf1 knockdown clones that significantly reduce Vezf1 protein levels upon treatment with doxycycline. We found that the knockdown of Vezf1 prior to mesoderm induction significantly impaired ESC differentiation but had no significant effect on cardiomyocyte development after mesoderm induction. These data indicate that Vezf1 expression is crucial for proper mesoderm and, thus, mesodermal lineage development. Further, FACS analysis showed reduced mesoderm cell populations derived from Vezf1 null post-differentiation. We used high throughput sequencing methods to determine genome-wide Vezf1 binding by ChIP-SEQ and compared gene expression in WT and Vezf1 null cells using RNA-SEQ. The data indicated that VEZF1 binds near the promoters of numerous Wnt signaling genes after differentiation and that the expression of Wnt pathway genes decreases when Vezf1 is lost. Interestingly, supplementing WNT3A protein in culture media of Vezf1 null cells rescues the expression of Wnt target genes necessary for mesoderm formation.
Differentiating Vezf1 KO cells to endothelial or cardiomyocyte lineages also resulted in massive cell death. The surviving cells interestingly stained positive for alkaline phosphatase (AP) staining, indicating retention of the pluripotency in Vezf1 KO cells. Whereas, re-culturing of WT ESC in LIF media, after differentiating them for five days in the absence of LIF, results in cell death, Vezf1 KO cells proliferate and form AP-positive and SSEA-positive colonies. We further show the retention of pluripotency gene expression post-differentiation using RNA sequencing and RT-qPCR. Moreover, we show that the continued expression of pluripotency genes post-differentiation was not a consequence of reduced global DNA methylation in Vezf1 KO cells.
Interestingly, our data show that Vezf1 is a transcriptional activator and binds to key pro-differentiation pathways like the MAPK signaling and WNT signaling pathways. The loss of Vezf1 correlates with reduced expression of genes in the pro-differentiation pathways. We show that CTCF, an insulator-binding protein, opportunistically binds to VEZF1 sites on genes in the pro-differentiation signaling pathways in VEZF1 KO cells. Therefore, we hypothesized that this opportunistic CTCF binding is the mechanism that drives the repression of pro-differentiation signaling genes or compensates for the loss of Vezf1 binding to support basal gene expression in the absence of VEZF1. Given the dire consequences of pluripotency in cancer stem cells, we investigated the expression of Vezf1 in cancers. We found that Vezf1 expression is reduced in many cancers and is correlated with poor prognosis. We also show that MAPK3, a prominent member of the MAPK signaling pathway, is reduced in these cancers, highlighting a strong correlation between Vezf1 expression and Mapk3 gene expression in cancers. The data extend our observation of pluripotency in ESCs to cancers. To gain further insights into the role of Vezf1 in cancer, we utilized F9 embryonic carcinoma cells. F9 cells have been reported to retain pluripotency expression post-differentiation. Interestingly, the ectopic and transient expression of Vezf1 in F9 cells significantly reduced the expression of pluripotency genes, suggesting that Vezf1 is sufficient to repress pluripotency gene expression in F9 carcinoma cells. These data highlight the significant role of Vezf1 in pluripotency gene repression and provide an excellent avenue for treating cancer relapse caused by the occurrence of cancer stem cells.
In conclusion, our research elucidates the critical role of Vezf1 in cardiomyocyte formation and pluripotency regulation during embryonic development. Understanding the molecular mechanisms underlying Vezf1-mediated pathways provides insights into developmental processes and holds promise for therapeutic interventions for cardiomyocyte regeneration and against cancers.
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Thursday, Jun 27, 2024 • Thomas Johns : [email protected]
Recently, Dr. Hanbo Shim Ph.D., University of Texas at Arlington Assistant Professor of Management, won the Academy of Management Human Resources Division Ralph Alexander Best Dissertation Awar d . The award will be presented at the 84th Annual Meeting of Academy of Management this August.
The AOM Human Resources Division is a division of the greater AOM organization which is dedicated to understanding and improving the efficiency and effectiveness of HR practices. The division is one of the AOM’s largest with over 3300 members. The division acts as a gathering place for academics and HR professionals to create networks and develop state-of-the-art HR knowledge.
This prestigious award is presented to research dissertations that have contributed to solving significant obstacles in the field of Human Resources.
“There’s an annual competition for all the best dissertations that isn’t bound to the state of Texas,” said Shim. “The competition encompasses dissertations that had been submitted within the last two years and you’re competing against everyone who submitted. They recognize the high quality of research and it gives you a goal to work toward.”
Dr. Hanbo Shim Ph.D., University of Texas at Arlington Assistant Professor of Management , poses for a photo at the UTA College of Business on June 25, 2024. (Photo by Jalen Larry)
Shim’s winning dissertation “Understanding the construct and measure of job performance over time”, delves into the ways employee job performance is captured at the short term and long-term levels.
“This study highlights the importance of more frequent or short-term performance feedback so that employees can make more effective behaviors decision on the short-term basis,” Shim explained. “It also informs individual employees about what would be the detail and ideal profile of short-term performance behaviors to win more favorable rating in the long-term.”
Through his research, Shim found that there was a deep correlation between short-term tasks and long-term employee ratings.
“Individual’s short-term performance behaviors such as performing his or her own tasks or helping others’ tasks can lead to different levels of long-term performance with different patterns,” said Shim. “This implies that the momentary decision that you make every day or every week in terms of how you work can create differences in your annual performance ratings.”
Through his dissertation, Shim hopes his findings and research will have a lasting effect on not only the Human Resources career field, but also the decision-making capabilities of leadership as a whole.
“This dissertation could have major implications for anyone in an HR field. Once you have more accurate and frequent data about employee performance, you’re able to make more timely and effective decisions.” Shim explained.
Although Shim was happy to receive this prestigious award, he views it as a successful team effort that allows the field of Human Resources to advance.
“I celebrated when I found out, not just with the people here at UTA, but with my fellow researchers like my dissertation director Dr. Michael Sturman at Rutgers.”, said Shim. “I felt joy, but I was also grateful to have the opportunity to give back. It feels great to find out that we can contribute something big to the organization and people we serve.”
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Thesis Order Form 2024. With over 30 years of experience, we specialise in providing high-quality thesis and dissertation binding services for universities and colleges in the United States. Our experienced team of binders can craft beautiful, professional-grade bindings for your thesis or dissertation. With our easy online thesis uploading ...
Mail-In your paper document for Binding Only. We can print your document from a PDF file (see sidebar), or you can mail your already printed document for Hard Cover Binding. Simply click your Hard Cover binding style above and follow the instructions. You will be prompted to print your order form. You'll then pack it with your documents and ...
This is the binding style that graduate students need in order to meet the requirements for a Doctorate at Princeton University. It is also attractive enough for presentation to advisors or undergraduate thesis submissions. Books up to ½-inch thick are made with a square (unrounded) spine. We round and back the spine for sizes above ½ an inch.
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Paperback binding ($10-20) Paperback binding is what is often used for real books. The pages are glued or sewn together, and your dissertation will be protected by a flexible cover. This type of binding makes your dissertation more durable and professional. In most cases, it's possible to add front and spine lettering.
Thesis Book Binding or Dissertation Book Binding is the conversion of your thesis or dissertation document into a printed and bound book format. Getting this done correctly involves several considerations that includes the Submission of your document for printing and binding, your choice of Paper, and the Style of binding you want.
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Thesis Order And Payment How do I go about ordering my thesis binding? Click through to the online order form for Thesis binding and printing.. How long does it take to print and bind my book? Due to the impact of COVID on our operation, our production time is now 18 business days from the next working day that we receive your order.
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Soft Cover Value Pricing. Our value pricing for soft cover thesis binding is $40 per book. Includes soft cover, we supply the cover stock. Choose black or white cover. With gold or silver foil stamping on spine and front cover. Printing Services. QBS also provides printing services on a variety of paper stocks. The prices are as follows:
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Thesis cover. $2.00/set. Thesis clips (small/medium) $.10. Thesis clips (large) . $.25. You can now order your thesis printing & binding online with our new site: printservices.mit.edu You put a great deal of work into your thesis. Now we can help you package your efforts with professional polish and style.
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Thesis binding is a specialised business so you should be wary of 'bookbinders' who have no experience binding theses. It is worth shopping around as costs can vary significantly. Some companies offer an online service. It is also a good idea to go to the library and check the inside covers of theses, to see if they contain the name of the ...
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Embryonic development relies on the complex interplay of epigenetic regulation, timely expression of genes, signal transduction pathways, and diverse morphological changes. The heart is the first organ to form during mammalian embryonic development. The proper development of the heart is critical to supply nutrients and oxygen to other cell types of the organism. Most cells that comprise the ...
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Recently, Dr. Hanbo Shim Ph.D., University of Texas at Arlington Assistant Professor of Management, won the Academy of Management Human Resources Division Ralph Alexander Best Dissertation Award. The AOM Human Resources Division is a division of the greater AOM organization which is dedicated to understanding and improving the efficiency and ...
From the "Total Before Shipping" detail summary, click "Submit Form" to proceed with your order. Choose shipping options and enter payment details. 3. Upload your PDF document and any images (for printed covers) that should accompany the file. With your order complete, your book (s) should ship in approximately one week.
In this thesis, I studied, through computational and experimental means, materials and therapeutics. Using molecular dynamics (MD) simulations, we examined: 1) dynamics of silver-alkaloid molecular motors, 2) bioinspired polymer separators, 3) amyloid inhibitors, 4) dendrimer-peptide conjugates, 5) phosphoethanolamine (pEtN) functionalized cellulose, and 6) synthesis of early-stage autophagy ...
Rovi has received non-binding offers for its third-party manufacturing business, the Spanish pharmaceuticals firm said on Wednesday, without disclosing the names of the bidders.
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The focus of this thesis is DAFS analysis on synthetic multi-Cu clusters, which are significant to biomimetic enzymatic sites or to be used in significant chemical transformations such as N2O and CO2 reduction. ... The close positioning of the metal centers enables them to collaborate in substrate binding and facilitate multielectron redox ...
Canada's federal government intervened on Thursday to prevent a strike by maintenance engineers at WestJet Airlines, the country's second-largest carrier, to avoid more flight cancellations ahead ...
(Reuters) -U.S. energy major Exxon Mobil said on Tuesday it had signed a non-binding agreement to supply lithium from its proposed Arkansas project to South Korean EV battery maker SK On. The ...
Thesis Binding at DB Bookbinders in Queensland is serious business, meaning we have the tools, staff, and experience to make your thesis or folio look perfect with a unique or classic design just for you. Choose from different cover types such as buckram, bonded leather, or genuine leather, and select from marbled end papers and bookmarks to ...