hypothesis testing about mean

Teach yourself statistics

Hypothesis Test for a Mean

This lesson explains how to conduct a hypothesis test of a mean, when the following conditions are met:

• The sampling method is simple random sampling .
• The sampling distribution is normal or nearly normal.

Generally, the sampling distribution will be approximately normally distributed if any of the following conditions apply.

• The population distribution is normal.
• The population distribution is symmetric , unimodal , without outliers , and the sample size is 15 or less.
• The population distribution is moderately skewed , unimodal, without outliers, and the sample size is between 16 and 40.
• The sample size is greater than 40, without outliers.

This approach consists of four steps: (1) state the hypotheses, (2) formulate an analysis plan, (3) analyze sample data, and (4) interpret results.

State the Hypotheses

Every hypothesis test requires the analyst to state a null hypothesis and an alternative hypothesis . The hypotheses are stated in such a way that they are mutually exclusive. That is, if one is true, the other must be false; and vice versa.

The table below shows three sets of hypotheses. Each makes a statement about how the population mean μ is related to a specified value M . (In the table, the symbol ≠ means " not equal to ".)

The first set of hypotheses (Set 1) is an example of a two-tailed test , since an extreme value on either side of the sampling distribution would cause a researcher to reject the null hypothesis. The other two sets of hypotheses (Sets 2 and 3) are one-tailed tests , since an extreme value on only one side of the sampling distribution would cause a researcher to reject the null hypothesis.

Formulate an Analysis Plan

The analysis plan describes how to use sample data to accept or reject the null hypothesis. It should specify the following elements.

• Significance level. Often, researchers choose significance levels equal to 0.01, 0.05, or 0.10; but any value between 0 and 1 can be used.
• Test method. Use the one-sample t-test to determine whether the hypothesized mean differs significantly from the observed sample mean.

Analyze Sample Data

Using sample data, conduct a one-sample t-test. This involves finding the standard error, degrees of freedom, test statistic, and the P-value associated with the test statistic.

SE = s * sqrt{ ( 1/n ) * [ ( N - n ) / ( N - 1 ) ] }

SE = s / sqrt( n )

• Degrees of freedom. The degrees of freedom (DF) is equal to the sample size (n) minus one. Thus, DF = n - 1.

t = ( x - μ) / SE

• P-value. The P-value is the probability of observing a sample statistic as extreme as the test statistic. Since the test statistic is a t statistic, use the t Distribution Calculator to assess the probability associated with the t statistic, given the degrees of freedom computed above. (See sample problems at the end of this lesson for examples of how this is done.)

Sample Size Calculator

As you probably noticed, the process of hypothesis testing can be complex. When you need to test a hypothesis about a mean score, consider using the Sample Size Calculator. The calculator is fairly easy to use, and it is free. You can find the Sample Size Calculator in Stat Trek's main menu under the Stat Tools tab. Or you can tap the button below.

Interpret Results

If the sample findings are unlikely, given the null hypothesis, the researcher rejects the null hypothesis. Typically, this involves comparing the P-value to the significance level , and rejecting the null hypothesis when the P-value is less than the significance level.

Test Your Understanding

In this section, two sample problems illustrate how to conduct a hypothesis test of a mean score. The first problem involves a two-tailed test; the second problem, a one-tailed test.

Problem 1: Two-Tailed Test

An inventor has developed a new, energy-efficient lawn mower engine. He claims that the engine will run continuously for 5 hours (300 minutes) on a single gallon of regular gasoline. From his stock of 2000 engines, the inventor selects a simple random sample of 50 engines for testing. The engines run for an average of 295 minutes, with a standard deviation of 20 minutes. Test the null hypothesis that the mean run time is 300 minutes against the alternative hypothesis that the mean run time is not 300 minutes. Use a 0.05 level of significance. (Assume that run times for the population of engines are normally distributed.)

Solution: The solution to this problem takes four steps: (1) state the hypotheses, (2) formulate an analysis plan, (3) analyze sample data, and (4) interpret results. We work through those steps below:

Null hypothesis: μ = 300

Alternative hypothesis: μ ≠ 300

• Formulate an analysis plan . For this analysis, the significance level is 0.05. The test method is a one-sample t-test .

SE = s / sqrt(n) = 20 / sqrt(50) = 20/7.07 = 2.83

DF = n - 1 = 50 - 1 = 49

t = ( x - μ) / SE = (295 - 300)/2.83 = -1.77

where s is the standard deviation of the sample, x is the sample mean, μ is the hypothesized population mean, and n is the sample size.

Since we have a two-tailed test , the P-value is the probability that the t statistic having 49 degrees of freedom is less than -1.77 or greater than 1.77. We use the t Distribution Calculator to find P(t < -1.77) is about 0.04.

• If you enter 1.77 as the sample mean in the t Distribution Calculator, you will find the that the P(t < 1.77) is about 0.04. Therefore, P(t >  1.77) is 1 minus 0.96 or 0.04. Thus, the P-value = 0.04 + 0.04 = 0.08.
• Interpret results . Since the P-value (0.08) is greater than the significance level (0.05), we cannot reject the null hypothesis.

Note: If you use this approach on an exam, you may also want to mention why this approach is appropriate. Specifically, the approach is appropriate because the sampling method was simple random sampling, the population was normally distributed, and the sample size was small relative to the population size (less than 5%).

Problem 2: One-Tailed Test

Bon Air Elementary School has 1000 students. The principal of the school thinks that the average IQ of students at Bon Air is at least 110. To prove her point, she administers an IQ test to 20 randomly selected students. Among the sampled students, the average IQ is 108 with a standard deviation of 10. Based on these results, should the principal accept or reject her original hypothesis? Assume a significance level of 0.01. (Assume that test scores in the population of engines are normally distributed.)

Null hypothesis: μ >= 110

Alternative hypothesis: μ < 110

• Formulate an analysis plan . For this analysis, the significance level is 0.01. The test method is a one-sample t-test .

SE = s / sqrt(n) = 10 / sqrt(20) = 10/4.472 = 2.236

DF = n - 1 = 20 - 1 = 19

t = ( x - μ) / SE = (108 - 110)/2.236 = -0.894

Here is the logic of the analysis: Given the alternative hypothesis (μ < 110), we want to know whether the observed sample mean is small enough to cause us to reject the null hypothesis.

The observed sample mean produced a t statistic test statistic of -0.894. We use the t Distribution Calculator to find P(t < -0.894) is about 0.19.

• This means we would expect to find a sample mean of 108 or smaller in 19 percent of our samples, if the true population IQ were 110. Thus the P-value in this analysis is 0.19.
• Interpret results . Since the P-value (0.19) is greater than the significance level (0.01), we cannot reject the null hypothesis.

Hypothesis Testing for Means & Proportions

Lisa Sullivan, PhD

Professor of Biostatistics

Boston University School of Public Health

Introduction

This is the first of three modules that will addresses the second area of statistical inference, which is hypothesis testing, in which a specific statement or hypothesis is generated about a population parameter, and sample statistics are used to assess the likelihood that the hypothesis is true. The hypothesis is based on available information and the investigator's belief about the population parameters. The process of hypothesis testing involves setting up two competing hypotheses, the null hypothesis and the alternate hypothesis. One selects a random sample (or multiple samples when there are more comparison groups), computes summary statistics and then assesses the likelihood that the sample data support the research or alternative hypothesis. Similar to estimation, the process of hypothesis testing is based on probability theory and the Central Limit Theorem.  

This module will focus on hypothesis testing for means and proportions. The next two modules in this series will address analysis of variance and chi-squared tests. 

Learning Objectives

After completing this module, the student will be able to:

• Define null and research hypothesis, test statistic, level of significance and decision rule
• Distinguish between Type I and Type II errors and discuss the implications of each
• Explain the difference between one and two sided tests of hypothesis
• Estimate and interpret p-values
• Explain the relationship between confidence interval estimates and p-values in drawing inferences
• Differentiate hypothesis testing procedures based on type of outcome variable and number of sample

Introduction to Hypothesis Testing

Techniques for hypothesis testing  .

The techniques for hypothesis testing depend on

• the type of outcome variable being analyzed (continuous, dichotomous, discrete)
• the number of comparison groups in the investigation
• whether the comparison groups are independent (i.e., physically separate such as men versus women) or dependent (i.e., matched or paired such as pre- and post-assessments on the same participants).

In estimation we focused explicitly on techniques for one and two samples and discussed estimation for a specific parameter (e.g., the mean or proportion of a population), for differences (e.g., difference in means, the risk difference) and ratios (e.g., the relative risk and odds ratio). Here we will focus on procedures for one and two samples when the outcome is either continuous (and we focus on means) or dichotomous (and we focus on proportions).

General Approach: A Simple Example

The Centers for Disease Control (CDC) reported on trends in weight, height and body mass index from the 1960's through 2002. 1 The general trend was that Americans were much heavier and slightly taller in 2002 as compared to 1960; both men and women gained approximately 24 pounds, on average, between 1960 and 2002.   In 2002, the mean weight for men was reported at 191 pounds. Suppose that an investigator hypothesizes that weights are even higher in 2006 (i.e., that the trend continued over the subsequent 4 years). The research hypothesis is that the mean weight in men in 2006 is more than 191 pounds. The null hypothesis is that there is no change in weight, and therefore the mean weight is still 191 pounds in 2006.  

In order to test the hypotheses, we select a random sample of American males in 2006 and measure their weights. Suppose we have resources available to recruit n=100 men into our sample. We weigh each participant and compute summary statistics on the sample data. Suppose in the sample we determine the following:

Do the sample data support the null or research hypothesis? The sample mean of 197.1 is numerically higher than 191. However, is this difference more than would be expected by chance? In hypothesis testing, we assume that the null hypothesis holds until proven otherwise. We therefore need to determine the likelihood of observing a sample mean of 197.1 or higher when the true population mean is 191 (i.e., if the null hypothesis is true or under the null hypothesis). We can compute this probability using the Central Limit Theorem. Specifically,

(Notice that we use the sample standard deviation in computing the Z score. This is generally an appropriate substitution as long as the sample size is large, n > 30. Thus, there is less than a 1% probability of observing a sample mean as large as 197.1 when the true population mean is 191. Do you think that the null hypothesis is likely true? Based on how unlikely it is to observe a sample mean of 197.1 under the null hypothesis (i.e., <1% probability), we might infer, from our data, that the null hypothesis is probably not true.

Suppose that the sample data had turned out differently. Suppose that we instead observed the following in 2006:

How likely it is to observe a sample mean of 192.1 or higher when the true population mean is 191 (i.e., if the null hypothesis is true)? We can again compute this probability using the Central Limit Theorem. Specifically,

There is a 33.4% probability of observing a sample mean as large as 192.1 when the true population mean is 191. Do you think that the null hypothesis is likely true?  

Neither of the sample means that we obtained allows us to know with certainty whether the null hypothesis is true or not. However, our computations suggest that, if the null hypothesis were true, the probability of observing a sample mean >197.1 is less than 1%. In contrast, if the null hypothesis were true, the probability of observing a sample mean >192.1 is about 33%. We can't know whether the null hypothesis is true, but the sample that provided a mean value of 197.1 provides much stronger evidence in favor of rejecting the null hypothesis, than the sample that provided a mean value of 192.1. Note that this does not mean that a sample mean of 192.1 indicates that the null hypothesis is true; it just doesn't provide compelling evidence to reject it.

In essence, hypothesis testing is a procedure to compute a probability that reflects the strength of the evidence (based on a given sample) for rejecting the null hypothesis. In hypothesis testing, we determine a threshold or cut-off point (called the critical value) to decide when to believe the null hypothesis and when to believe the research hypothesis. It is important to note that it is possible to observe any sample mean when the true population mean is true (in this example equal to 191), but some sample means are very unlikely. Based on the two samples above it would seem reasonable to believe the research hypothesis when x̄ = 197.1, but to believe the null hypothesis when x̄ =192.1. What we need is a threshold value such that if x̄ is above that threshold then we believe that H 1 is true and if x̄ is below that threshold then we believe that H 0 is true. The difficulty in determining a threshold for x̄ is that it depends on the scale of measurement. In this example, the threshold, sometimes called the critical value, might be 195 (i.e., if the sample mean is 195 or more then we believe that H 1 is true and if the sample mean is less than 195 then we believe that H 0 is true). Suppose we are interested in assessing an increase in blood pressure over time, the critical value will be different because blood pressures are measured in millimeters of mercury (mmHg) as opposed to in pounds. In the following we will explain how the critical value is determined and how we handle the issue of scale.

First, to address the issue of scale in determining the critical value, we convert our sample data (in particular the sample mean) into a Z score. We know from the module on probability that the center of the Z distribution is zero and extreme values are those that exceed 2 or fall below -2. Z scores above 2 and below -2 represent approximately 5% of all Z values. If the observed sample mean is close to the mean specified in H 0 (here m =191), then Z will be close to zero. If the observed sample mean is much larger than the mean specified in H 0 , then Z will be large.  

In hypothesis testing, we select a critical value from the Z distribution. This is done by first determining what is called the level of significance, denoted α ("alpha"). What we are doing here is drawing a line at extreme values. The level of significance is the probability that we reject the null hypothesis (in favor of the alternative) when it is actually true and is also called the Type I error rate.

α = Level of significance = P(Type I error) = P(Reject H 0 | H 0 is true).

Because α is a probability, it ranges between 0 and 1. The most commonly used value in the medical literature for α is 0.05, or 5%. Thus, if an investigator selects α=0.05, then they are allowing a 5% probability of incorrectly rejecting the null hypothesis in favor of the alternative when the null is in fact true. Depending on the circumstances, one might choose to use a level of significance of 1% or 10%. For example, if an investigator wanted to reject the null only if there were even stronger evidence than that ensured with α=0.05, they could choose a =0.01as their level of significance. The typical values for α are 0.01, 0.05 and 0.10, with α=0.05 the most commonly used value.  

Suppose in our weight study we select α=0.05. We need to determine the value of Z that holds 5% of the values above it (see below).

The critical value of Z for α =0.05 is Z = 1.645 (i.e., 5% of the distribution is above Z=1.645). With this value we can set up what is called our decision rule for the test. The rule is to reject H 0 if the Z score is 1.645 or more.  

With the first sample we have

Because 2.38 > 1.645, we reject the null hypothesis. (The same conclusion can be drawn by comparing the 0.0087 probability of observing a sample mean as extreme as 197.1 to the level of significance of 0.05. If the observed probability is smaller than the level of significance we reject H 0 ). Because the Z score exceeds the critical value, we conclude that the mean weight for men in 2006 is more than 191 pounds, the value reported in 2002. If we observed the second sample (i.e., sample mean =192.1), we would not be able to reject the null hypothesis because the Z score is 0.43 which is not in the rejection region (i.e., the region in the tail end of the curve above 1.645). With the second sample we do not have sufficient evidence (because we set our level of significance at 5%) to conclude that weights have increased. Again, the same conclusion can be reached by comparing probabilities. The probability of observing a sample mean as extreme as 192.1 is 33.4% which is not below our 5% level of significance.

Hypothesis Testing: Upper-, Lower, and Two Tailed Tests

The procedure for hypothesis testing is based on the ideas described above. Specifically, we set up competing hypotheses, select a random sample from the population of interest and compute summary statistics. We then determine whether the sample data supports the null or alternative hypotheses. The procedure can be broken down into the following five steps.  

• Step 1. Set up hypotheses and select the level of significance α.

H 0 : Null hypothesis (no change, no difference);  

H 1 : Research hypothesis (investigator's belief); α =0.05

• Step 2. Select the appropriate test statistic.  

The test statistic is a single number that summarizes the sample information.   An example of a test statistic is the Z statistic computed as follows:

When the sample size is small, we will use t statistics (just as we did when constructing confidence intervals for small samples). As we present each scenario, alternative test statistics are provided along with conditions for their appropriate use.

• Step 3.  Set up decision rule.  

The decision rule is a statement that tells under what circumstances to reject the null hypothesis. The decision rule is based on specific values of the test statistic (e.g., reject H 0 if Z > 1.645). The decision rule for a specific test depends on 3 factors: the research or alternative hypothesis, the test statistic and the level of significance. Each is discussed below.

• The decision rule depends on whether an upper-tailed, lower-tailed, or two-tailed test is proposed. In an upper-tailed test the decision rule has investigators reject H 0 if the test statistic is larger than the critical value. In a lower-tailed test the decision rule has investigators reject H 0 if the test statistic is smaller than the critical value.  In a two-tailed test the decision rule has investigators reject H 0 if the test statistic is extreme, either larger than an upper critical value or smaller than a lower critical value.
• The exact form of the test statistic is also important in determining the decision rule. If the test statistic follows the standard normal distribution (Z), then the decision rule will be based on the standard normal distribution. If the test statistic follows the t distribution, then the decision rule will be based on the t distribution. The appropriate critical value will be selected from the t distribution again depending on the specific alternative hypothesis and the level of significance.  
• The third factor is the level of significance. The level of significance which is selected in Step 1 (e.g., α =0.05) dictates the critical value.   For example, in an upper tailed Z test, if α =0.05 then the critical value is Z=1.645.  

The following figures illustrate the rejection regions defined by the decision rule for upper-, lower- and two-tailed Z tests with α=0.05. Notice that the rejection regions are in the upper, lower and both tails of the curves, respectively. The decision rules are written below each figure.

Rejection Region for Lower-Tailed Z Test (H 1 : μ < μ 0 ) with α =0.05

The decision rule is: Reject H 0 if Z < 1.645.

Rejection Region for Two-Tailed Z Test (H 1 : μ ≠ μ 0 ) with α =0.05

The decision rule is: Reject H 0 if Z < -1.960 or if Z > 1.960.

The complete table of critical values of Z for upper, lower and two-tailed tests can be found in the table of Z values to the right in "Other Resources."

Critical values of t for upper, lower and two-tailed tests can be found in the table of t values in "Other Resources."

• Step 4. Compute the test statistic.  

Here we compute the test statistic by substituting the observed sample data into the test statistic identified in Step 2.

• Step 5. Conclusion.  

The final conclusion is made by comparing the test statistic (which is a summary of the information observed in the sample) to the decision rule. The final conclusion will be either to reject the null hypothesis (because the sample data are very unlikely if the null hypothesis is true) or not to reject the null hypothesis (because the sample data are not very unlikely).  

If the null hypothesis is rejected, then an exact significance level is computed to describe the likelihood of observing the sample data assuming that the null hypothesis is true. The exact level of significance is called the p-value and it will be less than the chosen level of significance if we reject H 0 .

Statistical computing packages provide exact p-values as part of their standard output for hypothesis tests. In fact, when using a statistical computing package, the steps outlined about can be abbreviated. The hypotheses (step 1) should always be set up in advance of any analysis and the significance criterion should also be determined (e.g., α =0.05). Statistical computing packages will produce the test statistic (usually reporting the test statistic as t) and a p-value. The investigator can then determine statistical significance using the following: If p < α then reject H 0 .  

• Step 1. Set up hypotheses and determine level of significance

H 0 : μ = 191 H 1 : μ > 191                 α =0.05

The research hypothesis is that weights have increased, and therefore an upper tailed test is used.

• Step 2. Select the appropriate test statistic.

Because the sample size is large (n > 30) the appropriate test statistic is

• Step 3. Set up decision rule.  

In this example, we are performing an upper tailed test (H 1 : μ> 191), with a Z test statistic and selected α =0.05.   Reject H 0 if Z > 1.645.

We now substitute the sample data into the formula for the test statistic identified in Step 2.  

We reject H 0 because 2.38 > 1.645. We have statistically significant evidence at a =0.05, to show that the mean weight in men in 2006 is more than 191 pounds. Because we rejected the null hypothesis, we now approximate the p-value which is the likelihood of observing the sample data if the null hypothesis is true. An alternative definition of the p-value is the smallest level of significance where we can still reject H 0 . In this example, we observed Z=2.38 and for α=0.05, the critical value was 1.645. Because 2.38 exceeded 1.645 we rejected H 0 . In our conclusion we reported a statistically significant increase in mean weight at a 5% level of significance. Using the table of critical values for upper tailed tests, we can approximate the p-value. If we select α=0.025, the critical value is 1.96, and we still reject H 0 because 2.38 > 1.960. If we select α=0.010 the critical value is 2.326, and we still reject H 0 because 2.38 > 2.326. However, if we select α=0.005, the critical value is 2.576, and we cannot reject H 0 because 2.38 < 2.576. Therefore, the smallest α where we still reject H 0 is 0.010. This is the p-value. A statistical computing package would produce a more precise p-value which would be in between 0.005 and 0.010. Here we are approximating the p-value and would report p < 0.010.                  

Type I and Type II Errors

In all tests of hypothesis, there are two types of errors that can be committed. The first is called a Type I error and refers to the situation where we incorrectly reject H 0 when in fact it is true. This is also called a false positive result (as we incorrectly conclude that the research hypothesis is true when in fact it is not). When we run a test of hypothesis and decide to reject H 0 (e.g., because the test statistic exceeds the critical value in an upper tailed test) then either we make a correct decision because the research hypothesis is true or we commit a Type I error. The different conclusions are summarized in the table below. Note that we will never know whether the null hypothesis is really true or false (i.e., we will never know which row of the following table reflects reality).

Table - Conclusions in Test of Hypothesis

In the first step of the hypothesis test, we select a level of significance, α, and α= P(Type I error). Because we purposely select a small value for α, we control the probability of committing a Type I error. For example, if we select α=0.05, and our test tells us to reject H 0 , then there is a 5% probability that we commit a Type I error. Most investigators are very comfortable with this and are confident when rejecting H 0 that the research hypothesis is true (as it is the more likely scenario when we reject H 0 ).

When we run a test of hypothesis and decide not to reject H 0 (e.g., because the test statistic is below the critical value in an upper tailed test) then either we make a correct decision because the null hypothesis is true or we commit a Type II error. Beta (β) represents the probability of a Type II error and is defined as follows: β=P(Type II error) = P(Do not Reject H 0 | H 0 is false). Unfortunately, we cannot choose β to be small (e.g., 0.05) to control the probability of committing a Type II error because β depends on several factors including the sample size, α, and the research hypothesis. When we do not reject H 0 , it may be very likely that we are committing a Type II error (i.e., failing to reject H 0 when in fact it is false). Therefore, when tests are run and the null hypothesis is not rejected we often make a weak concluding statement allowing for the possibility that we might be committing a Type II error. If we do not reject H 0 , we conclude that we do not have significant evidence to show that H 1 is true. We do not conclude that H 0 is true.

 The most common reason for a Type II error is a small sample size.

Tests with One Sample, Continuous Outcome

Hypothesis testing applications with a continuous outcome variable in a single population are performed according to the five-step procedure outlined above. A key component is setting up the null and research hypotheses. The objective is to compare the mean in a single population to known mean (μ 0 ). The known value is generally derived from another study or report, for example a study in a similar, but not identical, population or a study performed some years ago. The latter is called a historical control. It is important in setting up the hypotheses in a one sample test that the mean specified in the null hypothesis is a fair and reasonable comparator. This will be discussed in the examples that follow.

Test Statistics for Testing H 0 : μ= μ 0

• if n > 30
• if n < 30

Note that statistical computing packages will use the t statistic exclusively and make the necessary adjustments for comparing the test statistic to appropriate values from probability tables to produce a p-value. 

The National Center for Health Statistics (NCHS) published a report in 2005 entitled Health, United States, containing extensive information on major trends in the health of Americans. Data are provided for the US population as a whole and for specific ages, sexes and races.  The NCHS report indicated that in 2002 Americans paid an average of $3,302 per year on health care and prescription drugs. An investigator hypothesizes that in 2005 expenditures have decreased primarily due to the availability of generic drugs. To test the hypothesis, a sample of 100 Americans are selected and their expenditures on health care and prescription drugs in 2005 are measured.   The sample data are summarized as follows: n=100, x̄

=$3,190 and s=$890. Is there statistical evidence of a reduction in expenditures on health care and prescription drugs in 2005? Is the sample mean of $3,190 evidence of a true reduction in the mean or is it within chance fluctuation? We will run the test using the five-step approach. 

• Step 1.  Set up hypotheses and determine level of significance

H 0 : μ = 3,302 H 1 : μ < 3,302           α =0.05

The research hypothesis is that expenditures have decreased, and therefore a lower-tailed test is used.

This is a lower tailed test, using a Z statistic and a 5% level of significance.   Reject H 0 if Z < -1.645.

•   Step 4. Compute the test statistic.  

We do not reject H 0 because -1.26 > -1.645. We do not have statistically significant evidence at α=0.05 to show that the mean expenditures on health care and prescription drugs are lower in 2005 than the mean of $3,302 reported in 2002.  

Recall that when we fail to reject H 0 in a test of hypothesis that either the null hypothesis is true (here the mean expenditures in 2005 are the same as those in 2002 and equal to $3,302) or we committed a Type II error (i.e., we failed to reject H 0 when in fact it is false). In summarizing this test, we conclude that we do not have sufficient evidence to reject H 0 . We do not conclude that H 0 is true, because there may be a moderate to high probability that we committed a Type II error. It is possible that the sample size is not large enough to detect a difference in mean expenditures.      

The NCHS reported that the mean total cholesterol level in 2002 for all adults was 203. Total cholesterol levels in participants who attended the seventh examination of the Offspring in the Framingham Heart Study are summarized as follows: n=3,310, x̄ =200.3, and s=36.8. Is there statistical evidence of a difference in mean cholesterol levels in the Framingham Offspring?

Here we want to assess whether the sample mean of 200.3 in the Framingham sample is statistically significantly different from 203 (i.e., beyond what we would expect by chance). We will run the test using the five-step approach.

H 0 : μ= 203 H 1 : μ≠ 203                       α=0.05

The research hypothesis is that cholesterol levels are different in the Framingham Offspring, and therefore a two-tailed test is used.

•   Step 3. Set up decision rule.  

This is a two-tailed test, using a Z statistic and a 5% level of significance. Reject H 0 if Z < -1.960 or is Z > 1.960.

We reject H 0 because -4.22 ≤ -1. .960. We have statistically significant evidence at α=0.05 to show that the mean total cholesterol level in the Framingham Offspring is different from the national average of 203 reported in 2002.   Because we reject H 0 , we also approximate a p-value. Using the two-sided significance levels, p < 0.0001.  

Statistical Significance versus Clinical (Practical) Significance

This example raises an important concept of statistical versus clinical or practical significance. From a statistical standpoint, the total cholesterol levels in the Framingham sample are highly statistically significantly different from the national average with p < 0.0001 (i.e., there is less than a 0.01% chance that we are incorrectly rejecting the null hypothesis). However, the sample mean in the Framingham Offspring study is 200.3, less than 3 units different from the national mean of 203. The reason that the data are so highly statistically significant is due to the very large sample size. It is always important to assess both statistical and clinical significance of data. This is particularly relevant when the sample size is large. Is a 3 unit difference in total cholesterol a meaningful difference?  

Consider again the NCHS-reported mean total cholesterol level in 2002 for all adults of 203. Suppose a new drug is proposed to lower total cholesterol. A study is designed to evaluate the efficacy of the drug in lowering cholesterol.   Fifteen patients are enrolled in the study and asked to take the new drug for 6 weeks. At the end of 6 weeks, each patient's total cholesterol level is measured and the sample statistics are as follows:   n=15, x̄ =195.9 and s=28.7. Is there statistical evidence of a reduction in mean total cholesterol in patients after using the new drug for 6 weeks? We will run the test using the five-step approach. 

H 0 : μ= 203 H 1 : μ< 203                   α=0.05

•  Step 2. Select the appropriate test statistic.  

Because the sample size is small (n<30) the appropriate test statistic is

This is a lower tailed test, using a t statistic and a 5% level of significance. In order to determine the critical value of t, we need degrees of freedom, df, defined as df=n-1. In this example df=15-1=14. The critical value for a lower tailed test with df=14 and a =0.05 is -2.145 and the decision rule is as follows:   Reject H 0 if t < -2.145.

We do not reject H 0 because -0.96 > -2.145. We do not have statistically significant evidence at α=0.05 to show that the mean total cholesterol level is lower than the national mean in patients taking the new drug for 6 weeks. Again, because we failed to reject the null hypothesis we make a weaker concluding statement allowing for the possibility that we may have committed a Type II error (i.e., failed to reject H 0 when in fact the drug is efficacious).

This example raises an important issue in terms of study design. In this example we assume in the null hypothesis that the mean cholesterol level is 203. This is taken to be the mean cholesterol level in patients without treatment. Is this an appropriate comparator? Alternative and potentially more efficient study designs to evaluate the effect of the new drug could involve two treatment groups, where one group receives the new drug and the other does not, or we could measure each patient's baseline or pre-treatment cholesterol level and then assess changes from baseline to 6 weeks post-treatment. These designs are also discussed here.

Video - Comparing a Sample Mean to Known Population Mean (8:20)

Link to transcript of the video

Tests with One Sample, Dichotomous Outcome

Hypothesis testing applications with a dichotomous outcome variable in a single population are also performed according to the five-step procedure. Similar to tests for means, a key component is setting up the null and research hypotheses. The objective is to compare the proportion of successes in a single population to a known proportion (p 0 ). That known proportion is generally derived from another study or report and is sometimes called a historical control. It is important in setting up the hypotheses in a one sample test that the proportion specified in the null hypothesis is a fair and reasonable comparator.    

In one sample tests for a dichotomous outcome, we set up our hypotheses against an appropriate comparator. We select a sample and compute descriptive statistics on the sample data. Specifically, we compute the sample size (n) and the sample proportion which is computed by taking the ratio of the number of successes to the sample size,

We then determine the appropriate test statistic (Step 2) for the hypothesis test. The formula for the test statistic is given below.

Test Statistic for Testing H 0 : p = p 0

if min(np 0 , n(1-p 0 )) > 5

The formula above is appropriate for large samples, defined when the smaller of np 0 and n(1-p 0 ) is at least 5. This is similar, but not identical, to the condition required for appropriate use of the confidence interval formula for a population proportion, i.e.,

Here we use the proportion specified in the null hypothesis as the true proportion of successes rather than the sample proportion. If we fail to satisfy the condition, then alternative procedures, called exact methods must be used to test the hypothesis about the population proportion.

Example:  

The NCHS report indicated that in 2002 the prevalence of cigarette smoking among American adults was 21.1%.  Data on prevalent smoking in n=3,536 participants who attended the seventh examination of the Offspring in the Framingham Heart Study indicated that 482/3,536 = 13.6% of the respondents were currently smoking at the time of the exam. Suppose we want to assess whether the prevalence of smoking is lower in the Framingham Offspring sample given the focus on cardiovascular health in that community. Is there evidence of a statistically lower prevalence of smoking in the Framingham Offspring study as compared to the prevalence among all Americans?

H 0 : p = 0.211 H 1 : p < 0.211                     α=0.05

We must first check that the sample size is adequate.   Specifically, we need to check min(np 0 , n(1-p 0 )) = min( 3,536(0.211), 3,536(1-0.211))=min(746, 2790)=746. The sample size is more than adequate so the following formula can be used:

This is a lower tailed test, using a Z statistic and a 5% level of significance. Reject H 0 if Z < -1.645.

We reject H 0 because -10.93 < -1.645. We have statistically significant evidence at α=0.05 to show that the prevalence of smoking in the Framingham Offspring is lower than the prevalence nationally (21.1%). Here, p < 0.0001.  

The NCHS report indicated that in 2002, 75% of children aged 2 to 17 saw a dentist in the past year. An investigator wants to assess whether use of dental services is similar in children living in the city of Boston. A sample of 125 children aged 2 to 17 living in Boston are surveyed and 64 reported seeing a dentist over the past 12 months. Is there a significant difference in use of dental services between children living in Boston and the national data?

Calculate this on your own before checking the answer.

Video - Hypothesis Test for One Sample and a Dichotomous Outcome (3:55)

Tests with Two Independent Samples, Continuous Outcome

There are many applications where it is of interest to compare two independent groups with respect to their mean scores on a continuous outcome. Here we compare means between groups, but rather than generating an estimate of the difference, we will test whether the observed difference (increase, decrease or difference) is statistically significant or not. Remember, that hypothesis testing gives an assessment of statistical significance, whereas estimation gives an estimate of effect and both are important.

Here we discuss the comparison of means when the two comparison groups are independent or physically separate. The two groups might be determined by a particular attribute (e.g., sex, diagnosis of cardiovascular disease) or might be set up by the investigator (e.g., participants assigned to receive an experimental treatment or placebo). The first step in the analysis involves computing descriptive statistics on each of the two samples. Specifically, we compute the sample size, mean and standard deviation in each sample and we denote these summary statistics as follows:

for sample 1:

for sample 2:

The designation of sample 1 and sample 2 is arbitrary. In a clinical trial setting the convention is to call the treatment group 1 and the control group 2. However, when comparing men and women, for example, either group can be 1 or 2.  

In the two independent samples application with a continuous outcome, the parameter of interest in the test of hypothesis is the difference in population means, μ 1 -μ 2 . The null hypothesis is always that there is no difference between groups with respect to means, i.e.,

The null hypothesis can also be written as follows: H 0 : μ 1 = μ 2 . In the research hypothesis, an investigator can hypothesize that the first mean is larger than the second (H 1 : μ 1 > μ 2 ), that the first mean is smaller than the second (H 1 : μ 1 < μ 2 ), or that the means are different (H 1 : μ 1 ≠ μ 2 ). The three different alternatives represent upper-, lower-, and two-tailed tests, respectively. The following test statistics are used to test these hypotheses.

Test Statistics for Testing H 0 : μ 1 = μ 2

• if n 1 > 30 and n 2 > 30
• if n 1 < 30 or n 2 < 30

NOTE: The formulas above assume equal variability in the two populations (i.e., the population variances are equal, or s 1 2 = s 2 2 ). This means that the outcome is equally variable in each of the comparison populations. For analysis, we have samples from each of the comparison populations. If the sample variances are similar, then the assumption about variability in the populations is probably reasonable. As a guideline, if the ratio of the sample variances, s 1 2 /s 2 2 is between 0.5 and 2 (i.e., if one variance is no more than double the other), then the formulas above are appropriate. If the ratio of the sample variances is greater than 2 or less than 0.5 then alternative formulas must be used to account for the heterogeneity in variances.    

The test statistics include Sp, which is the pooled estimate of the common standard deviation (again assuming that the variances in the populations are similar) computed as the weighted average of the standard deviations in the samples as follows:

Because we are assuming equal variances between groups, we pool the information on variability (sample variances) to generate an estimate of the variability in the population. Note: Because Sp is a weighted average of the standard deviations in the sample, Sp will always be in between s 1 and s 2 .)

Data measured on n=3,539 participants who attended the seventh examination of the Offspring in the Framingham Heart Study are shown below.  

Suppose we now wish to assess whether there is a statistically significant difference in mean systolic blood pressures between men and women using a 5% level of significance.  

H 0 : μ 1 = μ 2

H 1 : μ 1 ≠ μ 2                       α=0.05

Because both samples are large ( > 30), we can use the Z test statistic as opposed to t. Note that statistical computing packages use t throughout. Before implementing the formula, we first check whether the assumption of equality of population variances is reasonable. The guideline suggests investigating the ratio of the sample variances, s 1 2 /s 2 2 . Suppose we call the men group 1 and the women group 2. Again, this is arbitrary; it only needs to be noted when interpreting the results. The ratio of the sample variances is 17.5 2 /20.1 2 = 0.76, which falls between 0.5 and 2 suggesting that the assumption of equality of population variances is reasonable. The appropriate test statistic is

We now substitute the sample data into the formula for the test statistic identified in Step 2. Before substituting, we will first compute Sp, the pooled estimate of the common standard deviation.

Notice that the pooled estimate of the common standard deviation, Sp, falls in between the standard deviations in the comparison groups (i.e., 17.5 and 20.1). Sp is slightly closer in value to the standard deviation in the women (20.1) as there were slightly more women in the sample.   Recall, Sp is a weight average of the standard deviations in the comparison groups, weighted by the respective sample sizes.  

Now the test statistic:

We reject H 0 because 2.66 > 1.960. We have statistically significant evidence at α=0.05 to show that there is a difference in mean systolic blood pressures between men and women. The p-value is p < 0.010.  

Here again we find that there is a statistically significant difference in mean systolic blood pressures between men and women at p < 0.010. Notice that there is a very small difference in the sample means (128.2-126.5 = 1.7 units), but this difference is beyond what would be expected by chance. Is this a clinically meaningful difference? The large sample size in this example is driving the statistical significance. A 95% confidence interval for the difference in mean systolic blood pressures is: 1.7 + 1.26 or (0.44, 2.96). The confidence interval provides an assessment of the magnitude of the difference between means whereas the test of hypothesis and p-value provide an assessment of the statistical significance of the difference.  

Above we performed a study to evaluate a new drug designed to lower total cholesterol. The study involved one sample of patients, each patient took the new drug for 6 weeks and had their cholesterol measured. As a means of evaluating the efficacy of the new drug, the mean total cholesterol following 6 weeks of treatment was compared to the NCHS-reported mean total cholesterol level in 2002 for all adults of 203. At the end of the example, we discussed the appropriateness of the fixed comparator as well as an alternative study design to evaluate the effect of the new drug involving two treatment groups, where one group receives the new drug and the other does not. Here, we revisit the example with a concurrent or parallel control group, which is very typical in randomized controlled trials or clinical trials (refer to the EP713 module on Clinical Trials).  

A new drug is proposed to lower total cholesterol. A randomized controlled trial is designed to evaluate the efficacy of the medication in lowering cholesterol. Thirty participants are enrolled in the trial and are randomly assigned to receive either the new drug or a placebo. The participants do not know which treatment they are assigned. Each participant is asked to take the assigned treatment for 6 weeks. At the end of 6 weeks, each patient's total cholesterol level is measured and the sample statistics are as follows.

Is there statistical evidence of a reduction in mean total cholesterol in patients taking the new drug for 6 weeks as compared to participants taking placebo? We will run the test using the five-step approach.

H 0 : μ 1 = μ 2 H 1 : μ 1 < μ 2                         α=0.05

Because both samples are small (< 30), we use the t test statistic. Before implementing the formula, we first check whether the assumption of equality of population variances is reasonable. The ratio of the sample variances, s 1 2 /s 2 2 =28.7 2 /30.3 2 = 0.90, which falls between 0.5 and 2, suggesting that the assumption of equality of population variances is reasonable. The appropriate test statistic is:

This is a lower-tailed test, using a t statistic and a 5% level of significance. The appropriate critical value can be found in the t Table (in More Resources to the right). In order to determine the critical value of t we need degrees of freedom, df, defined as df=n 1 +n 2 -2 = 15+15-2=28. The critical value for a lower tailed test with df=28 and α=0.05 is -1.701 and the decision rule is: Reject H 0 if t < -1.701.

Now the test statistic,

We reject H 0 because -2.92 < -1.701. We have statistically significant evidence at α=0.05 to show that the mean total cholesterol level is lower in patients taking the new drug for 6 weeks as compared to patients taking placebo, p < 0.005.

The clinical trial in this example finds a statistically significant reduction in total cholesterol, whereas in the previous example where we had a historical control (as opposed to a parallel control group) we did not demonstrate efficacy of the new drug. Notice that the mean total cholesterol level in patients taking placebo is 217.4 which is very different from the mean cholesterol reported among all Americans in 2002 of 203 and used as the comparator in the prior example. The historical control value may not have been the most appropriate comparator as cholesterol levels have been increasing over time. In the next section, we present another design that can be used to assess the efficacy of the new drug.

Video - Comparison of Two Independent Samples With a Continuous Outcome (8:02)

Tests with Matched Samples, Continuous Outcome

In the previous section we compared two groups with respect to their mean scores on a continuous outcome. An alternative study design is to compare matched or paired samples. The two comparison groups are said to be dependent, and the data can arise from a single sample of participants where each participant is measured twice (possibly before and after an intervention) or from two samples that are matched on specific characteristics (e.g., siblings). When the samples are dependent, we focus on difference scores in each participant or between members of a pair and the test of hypothesis is based on the mean difference, μ d . The null hypothesis again reflects "no difference" and is stated as H 0 : μ d =0 . Note that there are some instances where it is of interest to test whether there is a difference of a particular magnitude (e.g., μ d =5) but in most instances the null hypothesis reflects no difference (i.e., μ d =0).  

The appropriate formula for the test of hypothesis depends on the sample size. The formulas are shown below and are identical to those we presented for estimating the mean of a single sample presented (e.g., when comparing against an external or historical control), except here we focus on difference scores.

Test Statistics for Testing H 0 : μ d =0

A new drug is proposed to lower total cholesterol and a study is designed to evaluate the efficacy of the drug in lowering cholesterol. Fifteen patients agree to participate in the study and each is asked to take the new drug for 6 weeks. However, before starting the treatment, each patient's total cholesterol level is measured. The initial measurement is a pre-treatment or baseline value. After taking the drug for 6 weeks, each patient's total cholesterol level is measured again and the data are shown below. The rightmost column contains difference scores for each patient, computed by subtracting the 6 week cholesterol level from the baseline level. The differences represent the reduction in total cholesterol over 4 weeks. (The differences could have been computed by subtracting the baseline total cholesterol level from the level measured at 6 weeks. The way in which the differences are computed does not affect the outcome of the analysis only the interpretation.)

Because the differences are computed by subtracting the cholesterols measured at 6 weeks from the baseline values, positive differences indicate reductions and negative differences indicate increases (e.g., participant 12 increases by 2 units over 6 weeks). The goal here is to test whether there is a statistically significant reduction in cholesterol. Because of the way in which we computed the differences, we want to look for an increase in the mean difference (i.e., a positive reduction). In order to conduct the test, we need to summarize the differences. In this sample, we have

The calculations are shown below.  

Is there statistical evidence of a reduction in mean total cholesterol in patients after using the new medication for 6 weeks? We will run the test using the five-step approach.

H 0 : μ d = 0 H 1 : μ d > 0                 α=0.05

NOTE: If we had computed differences by subtracting the baseline level from the level measured at 6 weeks then negative differences would have reflected reductions and the research hypothesis would have been H 1 : μ d < 0. 

• Step 2 . Select the appropriate test statistic.

This is an upper-tailed test, using a t statistic and a 5% level of significance. The appropriate critical value can be found in the t Table at the right, with df=15-1=14. The critical value for an upper-tailed test with df=14 and α=0.05 is 2.145 and the decision rule is Reject H 0 if t > 2.145.

We now substitute the sample data into the formula for the test statistic identified in Step 2.

We reject H 0 because 4.61 > 2.145. We have statistically significant evidence at α=0.05 to show that there is a reduction in cholesterol levels over 6 weeks.  

Here we illustrate the use of a matched design to test the efficacy of a new drug to lower total cholesterol. We also considered a parallel design (randomized clinical trial) and a study using a historical comparator. It is extremely important to design studies that are best suited to detect a meaningful difference when one exists. There are often several alternatives and investigators work with biostatisticians to determine the best design for each application. It is worth noting that the matched design used here can be problematic in that observed differences may only reflect a "placebo" effect. All participants took the assigned medication, but is the observed reduction attributable to the medication or a result of these participation in a study.

Video - Hypothesis Testing With a Matched Sample and a Continuous Outcome (3:11)

Tests with Two Independent Samples, Dichotomous Outcome

There are several approaches that can be used to test hypotheses concerning two independent proportions. Here we present one approach - the chi-square test of independence is an alternative, equivalent, and perhaps more popular approach to the same analysis. Hypothesis testing with the chi-square test is addressed in the third module in this series: BS704_HypothesisTesting-ChiSquare.

In tests of hypothesis comparing proportions between two independent groups, one test is performed and results can be interpreted to apply to a risk difference, relative risk or odds ratio. As a reminder, the risk difference is computed by taking the difference in proportions between comparison groups, the risk ratio is computed by taking the ratio of proportions, and the odds ratio is computed by taking the ratio of the odds of success in the comparison groups. Because the null values for the risk difference, the risk ratio and the odds ratio are different, the hypotheses in tests of hypothesis look slightly different depending on which measure is used. When performing tests of hypothesis for the risk difference, relative risk or odds ratio, the convention is to label the exposed or treated group 1 and the unexposed or control group 2.      

For example, suppose a study is designed to assess whether there is a significant difference in proportions in two independent comparison groups. The test of interest is as follows:

H 0 : p 1 = p 2 versus H 1 : p 1 ≠ p 2 .  

The following are the hypothesis for testing for a difference in proportions using the risk difference, the risk ratio and the odds ratio. First, the hypotheses above are equivalent to the following:

• For the risk difference, H 0 : p 1 - p 2 = 0 versus H 1 : p 1 - p 2 ≠ 0 which are, by definition, equal to H 0 : RD = 0 versus H 1 : RD ≠ 0.
• If an investigator wants to focus on the risk ratio, the equivalent hypotheses are H 0 : RR = 1 versus H 1 : RR ≠ 1.
• If the investigator wants to focus on the odds ratio, the equivalent hypotheses are H 0 : OR = 1 versus H 1 : OR ≠ 1.  

Suppose a test is performed to test H 0 : RD = 0 versus H 1 : RD ≠ 0 and the test rejects H 0 at α=0.05. Based on this test we can conclude that there is significant evidence, α=0.05, of a difference in proportions, significant evidence that the risk difference is not zero, significant evidence that the risk ratio and odds ratio are not one. The risk difference is analogous to the difference in means when the outcome is continuous. Here the parameter of interest is the difference in proportions in the population, RD = p 1 -p 2 and the null value for the risk difference is zero. In a test of hypothesis for the risk difference, the null hypothesis is always H 0 : RD = 0. This is equivalent to H 0 : RR = 1 and H 0 : OR = 1. In the research hypothesis, an investigator can hypothesize that the first proportion is larger than the second (H 1 : p 1 > p 2 , which is equivalent to H 1 : RD > 0, H 1 : RR > 1 and H 1 : OR > 1), that the first proportion is smaller than the second (H 1 : p 1 < p 2 , which is equivalent to H 1 : RD < 0, H 1 : RR < 1 and H 1 : OR < 1), or that the proportions are different (H 1 : p 1 ≠ p 2 , which is equivalent to H 1 : RD ≠ 0, H 1 : RR ≠ 1 and H 1 : OR ≠

1). The three different alternatives represent upper-, lower- and two-tailed tests, respectively.  

The formula for the test of hypothesis for the difference in proportions is given below.

Test Statistics for Testing H 0 : p 1 = p

The formula above is appropriate for large samples, defined as at least 5 successes (np > 5) and at least 5 failures (n(1-p > 5)) in each of the two samples. If there are fewer than 5 successes or failures in either comparison group, then alternative procedures, called exact methods must be used to estimate the difference in population proportions.

The following table summarizes data from n=3,799 participants who attended the fifth examination of the Offspring in the Framingham Heart Study. The outcome of interest is prevalent CVD and we want to test whether the prevalence of CVD is significantly higher in smokers as compared to non-smokers.

The prevalence of CVD (or proportion of participants with prevalent CVD) among non-smokers is 298/3,055 = 0.0975 and the prevalence of CVD among current smokers is 81/744 = 0.1089. Here smoking status defines the comparison groups and we will call the current smokers group 1 (exposed) and the non-smokers (unexposed) group 2. The test of hypothesis is conducted below using the five step approach.

H 0 : p 1 = p 2     H 1 : p 1 ≠ p 2                 α=0.05

• Step 2.  Select the appropriate test statistic.  

We must first check that the sample size is adequate. Specifically, we need to ensure that we have at least 5 successes and 5 failures in each comparison group. In this example, we have more than enough successes (cases of prevalent CVD) and failures (persons free of CVD) in each comparison group. The sample size is more than adequate so the following formula can be used:

Reject H 0 if Z < -1.960 or if Z > 1.960.

We now substitute the sample data into the formula for the test statistic identified in Step 2. We first compute the overall proportion of successes:

We now substitute to compute the test statistic.

• Step 5. Conclusion.

We do not reject H 0 because -1.960 < 0.927 < 1.960. We do not have statistically significant evidence at α=0.05 to show that there is a difference in prevalent CVD between smokers and non-smokers.  

A 95% confidence interval for the difference in prevalent CVD (or risk difference) between smokers and non-smokers as 0.0114 + 0.0247, or between -0.0133 and 0.0361. Because the 95% confidence interval for the risk difference includes zero we again conclude that there is no statistically significant difference in prevalent CVD between smokers and non-smokers.    

Smoking has been shown over and over to be a risk factor for cardiovascular disease. What might explain the fact that we did not observe a statistically significant difference using data from the Framingham Heart Study? HINT: Here we consider prevalent CVD, would the results have been different if we considered incident CVD?

A randomized trial is designed to evaluate the effectiveness of a newly developed pain reliever designed to reduce pain in patients following joint replacement surgery. The trial compares the new pain reliever to the pain reliever currently in use (called the standard of care). A total of 100 patients undergoing joint replacement surgery agreed to participate in the trial. Patients were randomly assigned to receive either the new pain reliever or the standard pain reliever following surgery and were blind to the treatment assignment. Before receiving the assigned treatment, patients were asked to rate their pain on a scale of 0-10 with higher scores indicative of more pain. Each patient was then given the assigned treatment and after 30 minutes was again asked to rate their pain on the same scale. The primary outcome was a reduction in pain of 3 or more scale points (defined by clinicians as a clinically meaningful reduction). The following data were observed in the trial.

We now test whether there is a statistically significant difference in the proportions of patients reporting a meaningful reduction (i.e., a reduction of 3 or more scale points) using the five step approach.  

H 0 : p 1 = p 2     H 1 : p 1 ≠ p 2              α=0.05

Here the new or experimental pain reliever is group 1 and the standard pain reliever is group 2.

We must first check that the sample size is adequate. Specifically, we need to ensure that we have at least 5 successes and 5 failures in each comparison group, i.e.,

In this example, we have min(50(0.46), 50(1-0.46), 50(0.22), 50(1-0.22)) = min(23, 27, 11, 39) = 11. The sample size is adequate so the following formula can be used

We reject H 0 because 2.526 > 1960. We have statistically significant evidence at a =0.05 to show that there is a difference in the proportions of patients on the new pain reliever reporting a meaningful reduction (i.e., a reduction of 3 or more scale points) as compared to patients on the standard pain reliever.

A 95% confidence interval for the difference in proportions of patients on the new pain reliever reporting a meaningful reduction (i.e., a reduction of 3 or more scale points) as compared to patients on the standard pain reliever is 0.24 + 0.18 or between 0.06 and 0.42. Because the 95% confidence interval does not include zero we concluded that there was a statistically significant difference in proportions which is consistent with the test of hypothesis result. 

Again, the procedures discussed here apply to applications where there are two independent comparison groups and a dichotomous outcome. There are other applications in which it is of interest to compare a dichotomous outcome in matched or paired samples. For example, in a clinical trial we might wish to test the effectiveness of a new antibiotic eye drop for the treatment of bacterial conjunctivitis. Participants use the new antibiotic eye drop in one eye and a comparator (placebo or active control treatment) in the other. The success of the treatment (yes/no) is recorded for each participant for each eye. Because the two assessments (success or failure) are paired, we cannot use the procedures discussed here. The appropriate test is called McNemar's test (sometimes called McNemar's test for dependent proportions).  

Vide0 - Hypothesis Testing With Two Independent Samples and a Dichotomous Outcome (2:55)

Here we presented hypothesis testing techniques for means and proportions in one and two sample situations. Tests of hypothesis involve several steps, including specifying the null and alternative or research hypothesis, selecting and computing an appropriate test statistic, setting up a decision rule and drawing a conclusion. There are many details to consider in hypothesis testing. The first is to determine the appropriate test. We discussed Z and t tests here for different applications. The appropriate test depends on the distribution of the outcome variable (continuous or dichotomous), the number of comparison groups (one, two) and whether the comparison groups are independent or dependent. The following table summarizes the different tests of hypothesis discussed here.

• Continuous Outcome, One Sample: H0: μ = μ0
• Continuous Outcome, Two Independent Samples: H0: μ1 = μ2
• Continuous Outcome, Two Matched Samples: H0: μd = 0
• Dichotomous Outcome, One Sample: H0: p = p 0
• Dichotomous Outcome, Two Independent Samples: H0: p1 = p2, RD=0, RR=1, OR=1

Once the type of test is determined, the details of the test must be specified. Specifically, the null and alternative hypotheses must be clearly stated. The null hypothesis always reflects the "no change" or "no difference" situation. The alternative or research hypothesis reflects the investigator's belief. The investigator might hypothesize that a parameter (e.g., a mean, proportion, difference in means or proportions) will increase, will decrease or will be different under specific conditions (sometimes the conditions are different experimental conditions and other times the conditions are simply different groups of participants). Once the hypotheses are specified, data are collected and summarized. The appropriate test is then conducted according to the five step approach. If the test leads to rejection of the null hypothesis, an approximate p-value is computed to summarize the significance of the findings. When tests of hypothesis are conducted using statistical computing packages, exact p-values are computed. Because the statistical tables in this textbook are limited, we can only approximate p-values. If the test fails to reject the null hypothesis, then a weaker concluding statement is made for the following reason.

In hypothesis testing, there are two types of errors that can be committed. A Type I error occurs when a test incorrectly rejects the null hypothesis. This is referred to as a false positive result, and the probability that this occurs is equal to the level of significance, α. The investigator chooses the level of significance in Step 1, and purposely chooses a small value such as α=0.05 to control the probability of committing a Type I error. A Type II error occurs when a test fails to reject the null hypothesis when in fact it is false. The probability that this occurs is equal to β. Unfortunately, the investigator cannot specify β at the outset because it depends on several factors including the sample size (smaller samples have higher b), the level of significance (β decreases as a increases), and the difference in the parameter under the null and alternative hypothesis.    

We noted in several examples in this chapter, the relationship between confidence intervals and tests of hypothesis. The approaches are different, yet related. It is possible to draw a conclusion about statistical significance by examining a confidence interval. For example, if a 95% confidence interval does not contain the null value (e.g., zero when analyzing a mean difference or risk difference, one when analyzing relative risks or odds ratios), then one can conclude that a two-sided test of hypothesis would reject the null at α=0.05. It is important to note that the correspondence between a confidence interval and test of hypothesis relates to a two-sided test and that the confidence level corresponds to a specific level of significance (e.g., 95% to α=0.05, 90% to α=0.10 and so on). The exact significance of the test, the p-value, can only be determined using the hypothesis testing approach and the p-value provides an assessment of the strength of the evidence and not an estimate of the effect.

Answers to Selected Problems

Dental services problem - bottom of page 5.

• Step 1: Set up hypotheses and determine the level of significance.

α=0.05

• Step 2: Select the appropriate test statistic.

First, determine whether the sample size is adequate.

Therefore the sample size is adequate, and we can use the following formula:

• Step 3: Set up the decision rule.

Reject H0 if Z is less than or equal to -1.96 or if Z is greater than or equal to 1.96.

• Step 4: Compute the test statistic
• Step 5: Conclusion.

We reject the null hypothesis because -6.15<-1.96. Therefore there is a statistically significant difference in the proportion of children in Boston using dental services compated to the national proportion.

• FOR INSTRUCTOR
• FOR INSTRUCTORS

8.4.3 Hypothesis Testing for the Mean

$\quad$ $H_0$: $\mu=\mu_0$, $\quad$ $H_1$: $\mu \neq \mu_0$.

$\quad$ $H_0$: $\mu \leq \mu_0$, $\quad$ $H_1$: $\mu > \mu_0$.

$\quad$ $H_0$: $\mu \geq \mu_0$, $\quad$ $H_1$: $\mu \lt \mu_0$.

Two-sided Tests for the Mean:

Therefore, we can suggest the following test. Choose a threshold, and call it $c$. If $|W| \leq c$, accept $H_0$, and if $|W|>c$, accept $H_1$. How do we choose $c$? If $\alpha$ is the required significance level, we must have

• As discussed above, we let \begin{align}%\label{} W(X_1,X_2, \cdots,X_n)=\frac{\overline{X}-\mu_0}{\sigma / \sqrt{n}}. \end{align} Note that, assuming $H_0$, $W \sim N(0,1)$. We will choose a threshold, $c$. If $|W| \leq c$, we accept $H_0$, and if $|W|>c$, accept $H_1$. To choose $c$, we let \begin{align} P(|W| > c \; | \; H_0) =\alpha. \end{align} Since the standard normal PDF is symmetric around $0$, we have \begin{align} P(|W| > c \; | \; H_0) = 2 P(W>c | \; H_0). \end{align} Thus, we conclude $P(W>c | \; H_0)=\frac{\alpha}{2}$. Therefore, \begin{align} c=z_{\frac{\alpha}{2}}. \end{align} Therefore, we accept $H_0$ if \begin{align} \left|\frac{\overline{X}-\mu_0}{\sigma / \sqrt{n}} \right| \leq z_{\frac{\alpha}{2}}, \end{align} and reject it otherwise.
• We have \begin{align} \beta (\mu) &=P(\textrm{type II error}) = P(\textrm{accept }H_0 \; | \; \mu) \\ &= P\left(\left|\frac{\overline{X}-\mu_0}{\sigma / \sqrt{n}} \right| \lt z_{\frac{\alpha}{2}}\; | \; \mu \right). \end{align} If $X_i \sim N(\mu,\sigma^2)$, then $\overline{X} \sim N(\mu, \frac{\sigma^2}{n})$. Thus, \begin{align} \beta (\mu)&=P\left(\left|\frac{\overline{X}-\mu_0}{\sigma / \sqrt{n}} \right| \lt z_{\frac{\alpha}{2}}\; | \; \mu \right)\\ &=P\left(\mu_0- z_{\frac{\alpha}{2}} \frac{\sigma}{\sqrt{n}} \leq \overline{X} \leq \mu_0+ z_{\frac{\alpha}{2}} \frac{\sigma}{\sqrt{n}}\right)\\ &=\Phi\left(z_{\frac{\alpha}{2}}+\frac{\mu_0-\mu}{\sigma / \sqrt{n}}\right)-\Phi\left(-z_{\frac{\alpha}{2}}+\frac{\mu_0-\mu}{\sigma / \sqrt{n}}\right). \end{align}
• Let $S^2$ be the sample variance for this random sample. Then, the random variable $W$ defined as \begin{equation} W(X_1,X_2, \cdots, X_n)=\frac{\overline{X}-\mu_0}{S / \sqrt{n}} \end{equation} has a $t$-distribution with $n-1$ degrees of freedom, i.e., $W \sim T(n-1)$. Thus, we can repeat the analysis of Example 8.24 here. The only difference is that we need to replace $\sigma$ by $S$ and $z_{\frac{\alpha}{2}}$ by $t_{\frac{\alpha}{2},n-1}$. Therefore, we accept $H_0$ if \begin{align} |W| \leq t_{\frac{\alpha}{2},n-1}, \end{align} and reject it otherwise. Let us look at a numerical example of this case.

$\quad$ $H_0$: $\mu=170$, $\quad$ $H_1$: $\mu \neq 170$.

• Let's first compute the sample mean and the sample standard deviation. The sample mean is \begin{align}%\label{} \overline{X}&=\frac{X_1+X_2+X_3+X_4+X_5+X_6+X_7+X_8+X_9}{9}\\ &=165.8 \end{align} The sample variance is given by \begin{align}%\label{} {S}^2=\frac{1}{9-1} \sum_{k=1}^9 (X_k-\overline{X})^2&=68.01 \end{align} The sample standard deviation is given by \begin{align}%\label{} S&= \sqrt{S^2}=8.25 \end{align} The following MATLAB code can be used to obtain these values: x=[176.2,157.9,160.1,180.9,165.1,167.2,162.9,155.7,166.2]; m=mean(x); v=var(x); s=std(x); Now, our test statistic is \begin{align} W(X_1,X_2, \cdots, X_9)&=\frac{\overline{X}-\mu_0}{S / \sqrt{n}}\\ &=\frac{165.8-170}{8.25 / 3}=-1.52 \end{align} Thus, $|W|=1.52$. Also, we have \begin{align} t_{\frac{\alpha}{2},n-1} = t_{0.025,8} \approx 2.31 \end{align} The above value can be obtained in MATLAB using the command $\mathtt{tinv(0.975,8)}$. Thus, we conclude \begin{align} |W| \leq t_{\frac{\alpha}{2},n-1}. \end{align} Therefore, we accept $H_0$. In other words, we do not have enough evidence to conclude that the average height in the city is different from the average height in the country.

Let us summarize what we have obtained for the two-sided test for the mean.

One-sided Tests for the Mean:

• As before, we define the test statistic as \begin{align}%\label{} W(X_1,X_2, \cdots,X_n)=\frac{\overline{X}-\mu_0}{\sigma / \sqrt{n}}. \end{align} If $H_0$ is true (i.e., $\mu \leq \mu_0$), we expect $\overline{X}$ (and thus $W$) to be relatively small, while if $H_1$ is true, we expect $\overline{X}$ (and thus $W$) to be larger. This suggests the following test: Choose a threshold, and call it $c$. If $W \leq c$, accept $H_0$, and if $W>c$, accept $H_1$. How do we choose $c$? If $\alpha$ is the required significance level, we must have \begin{align} P(\textrm{type I error}) &= P(\textrm{Reject }H_0 \; | \; H_0) \\ &= P(W > c \; | \; \mu \leq \mu_0) \leq \alpha. \end{align} Here, the probability of type I error depends on $\mu$. More specifically, for any $\mu \leq \mu_0$, we can write \begin{align} P(\textrm{type I error} \; | \; \mu) &= P(\textrm{Reject }H_0 \; | \; \mu) \\ &= P(W > c \; | \; \mu)\\ &=P \left(\frac{\overline{X}-\mu_0}{\sigma / \sqrt{n}}> c \; | \; \mu\right)\\ &=P \left(\frac{\overline{X}-\mu}{\sigma / \sqrt{n}}+\frac{\mu-\mu_0}{\sigma / \sqrt{n}}> c \; | \; \mu\right)\\ &=P \left(\frac{\overline{X}-\mu}{\sigma / \sqrt{n}}> c+\frac{\mu_0-\mu}{\sigma / \sqrt{n}} \; | \; \mu\right)\\ &\leq P \left(\frac{\overline{X}-\mu}{\sigma / \sqrt{n}}> c \; | \; \mu\right) \quad (\textrm{ since }\mu \leq \mu_0)\\ &=1-\Phi(c) \quad \big(\textrm{ since given }\mu, \frac{\overline{X}-\mu}{\sigma / \sqrt{n}} \sim N(0,1) \big). \end{align} Thus, we can choose $\alpha=1-\Phi(c)$, which results in \begin{align} c=z_{\alpha}. \end{align} Therefore, we accept $H_0$ if \begin{align} \frac{\overline{X}-\mu_0}{\sigma / \sqrt{n}} \leq z_{\alpha}, \end{align} and reject it otherwise.

$\quad$ $H_0$: $\mu \geq \mu_0$, $\quad$ $H_1$: $\mu \lt \mu_0$,

Hypothesis tests about the mean

by Marco Taboga , PhD

This lecture explains how to conduct hypothesis tests about the mean of a normal distribution.

We tackle two different cases:

when we know the variance of the distribution, then we use a z-statistic to conduct the test;

when the variance is unknown, then we use the t-statistic.

In each case we derive the power and the size of the test.

We conclude with two solved exercises on size and power.

Table of contents

Known variance: the z-test

The null hypothesis, the test statistic, the critical region, the decision, the power function, the size of the test, how to choose the critical value, unknown variance: the t-test, how to choose the critical values, solved exercises.

The assumptions are the same we made in the lecture on confidence intervals for the mean .

A test of hypothesis based on it is called z-test .

Otherwise, it is not rejected.

We explain how to do this in the page on critical values .

This case is similar to the previous one. The only difference is that we now relax the assumption that the variance of the distribution is known.

The test of hypothesis based on it is called t-test .

Otherwise, we do not reject it.

The page on critical values explains how this equation is solved.

Below you can find some exercises with explained solutions.

Suppose that a statistician observes 100 independent realizations of a normal random variable.

The mean and the variance of the random variable, which the statistician does not know, are equal to 1 and 4 respectively.

Find the probability that the statistician will reject the null hypothesis that the mean is equal to zero if:

she runs a t-test based on the 100 observed realizations;

A statistician observes 100 independent realizations of a normal random variable.

She performs a t-test of the null hypothesis that the mean of the variable is equal to zero.

How to cite

Please cite as:

Taboga, Marco (2021). "Hypothesis tests about the mean", Lectures on probability theory and mathematical statistics. Kindle Direct Publishing. Online appendix. https://www.statlect.com/fundamentals-of-statistics/hypothesis-testing-mean.

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8.6: Hypothesis Test of a Single Population Mean with Examples

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Steps for performing Hypothesis Test of a Single Population Mean

Step 1: State your hypotheses about the population mean. Step 2: Summarize the data. State a significance level. State and check conditions required for the procedure

• Find or identify the sample size, n, the sample mean, \(\bar{x}\) and the sample standard deviation, s .

The sampling distribution for the one-mean test statistic is, approximately, T- distribution if the following conditions are met

• Sample is random with independent observations .
• Sample is large. The population must be Normal or the sample size must be at least 30.

Step 3: Perform the procedure based on the assumption that \(H_{0}\) is true

• Find the Estimated Standard Error: \(SE=\frac{s}{\sqrt{n}}\).
• Compute the observed value of the test statistic: \(T_{obs}=\frac{\bar{x}-\mu_{0}}{SE}\).
• Check the type of the test (right-, left-, or two-tailed)
• Find the p-value in order to measure your level of surprise.

Step 4: Make a decision about \(H_{0}\) and \(H_{a}\)

• Do you reject or not reject your null hypothesis?

Step 5: Make a conclusion

• What does this mean in the context of the data?

The following examples illustrate a left-, right-, and two-tailed test.

Example \(\pageindex{1}\).

\(H_{0}: \mu = 5, H_{a}: \mu < 5\)

Test of a single population mean. \(H_{a}\) tells you the test is left-tailed. The picture of the \(p\)-value is as follows:

Exercise \(\PageIndex{1}\)

\(H_{0}: \mu = 10, H_{a}: \mu < 10\)

Assume the \(p\)-value is 0.0935. What type of test is this? Draw the picture of the \(p\)-value.

left-tailed test

Example \(\PageIndex{2}\)

\(H_{0}: \mu \leq 0.2, H_{a}: \mu > 0.2\)

This is a test of a single population proportion. \(H_{a}\) tells you the test is right-tailed . The picture of the p -value is as follows:

Exercise \(\PageIndex{2}\)

\(H_{0}: \mu \leq 1, H_{a}: \mu > 1\)

Assume the \(p\)-value is 0.1243. What type of test is this? Draw the picture of the \(p\)-value.

right-tailed test

Example \(\PageIndex{3}\)

\(H_{0}: \mu = 50, H_{a}: \mu \neq 50\)

This is a test of a single population mean. \(H_{a}\) tells you the test is two-tailed . The picture of the \(p\)-value is as follows.

Exercise \(\PageIndex{3}\)

\(H_{0}: \mu = 0.5, H_{a}: \mu \neq 0.5\)

Assume the p -value is 0.2564. What type of test is this? Draw the picture of the \(p\)-value.

two-tailed test

Full Hypothesis Test Examples

Example \(\pageindex{4}\).

Statistics students believe that the mean score on the first statistics test is 65. A statistics instructor thinks the mean score is higher than 65. He samples ten statistics students and obtains the scores 65 65 70 67 66 63 63 68 72 71. He performs a hypothesis test using a 5% level of significance. The data are assumed to be from a normal distribution.

Set up the hypothesis test:

A 5% level of significance means that \(\alpha = 0.05\). This is a test of a single population mean .

\(H_{0}: \mu = 65  H_{a}: \mu > 65\)

Since the instructor thinks the average score is higher, use a "\(>\)". The "\(>\)" means the test is right-tailed.

Determine the distribution needed:

Random variable: \(\bar{X} =\) average score on the first statistics test.

Distribution for the test: If you read the problem carefully, you will notice that there is no population standard deviation given . You are only given \(n = 10\) sample data values. Notice also that the data come from a normal distribution. This means that the distribution for the test is a student's \(t\).

Use \(t_{df}\). Therefore, the distribution for the test is \(t_{9}\) where \(n = 10\) and \(df = 10 - 1 = 9\).

The sample mean and sample standard deviation are calculated as 67 and 3.1972 from the data.

Calculate the \(p\)-value using the Student's \(t\)-distribution:

\[t_{obs} = \dfrac{\bar{x}-\mu_{\bar{x}}}{\left(\dfrac{s}{\sqrt{n}}\right)}=\dfrac{67-65}{\left(\dfrac{3.1972}{\sqrt{10}}\right)}\]

Use the T-table or Excel's t_dist() function to find p-value:

\(p\text{-value} = P(\bar{x} > 67) =P(T >1.9782 )= 1-0.9604=0.0396\)

Interpretation of the p -value: If the null hypothesis is true, then there is a 0.0396 probability (3.96%) that the sample mean is 65 or more.

Compare \(\alpha\) and the \(p-\text{value}\):

Since \(α = 0.05\) and \(p\text{-value} = 0.0396\). \(\alpha > p\text{-value}\).

Make a decision: Since \(\alpha > p\text{-value}\), reject \(H_{0}\).

This means you reject \(\mu = 65\). In other words, you believe the average test score is more than 65.

Conclusion: At a 5% level of significance, the sample data show sufficient evidence that the mean (average) test score is more than 65, just as the math instructor thinks.

The \(p\text{-value}\) can easily be calculated.

Put the data into a list. Press STAT and arrow over to TESTS . Press 2:T-Test . Arrow over to Data and press ENTER . Arrow down and enter 65 for \(\mu_{0}\), the name of the list where you put the data, and 1 for Freq: . Arrow down to \(\mu\): and arrow over to \(> \mu_{0}\). Press ENTER . Arrow down to Calculate and press ENTER . The calculator not only calculates the \(p\text{-value}\) (p = 0.0396) but it also calculates the test statistic ( t -score) for the sample mean, the sample mean, and the sample standard deviation. \(\mu > 65\) is the alternative hypothesis. Do this set of instructions again except arrow to Draw (instead of Calculate ). Press ENTER . A shaded graph appears with \(t = 1.9781\) (test statistic) and \(p = 0.0396\) (\(p\text{-value}\)). Make sure when you use Draw that no other equations are highlighted in \(Y =\) and the plots are turned off.

Exercise \(\PageIndex{4}\)

It is believed that a stock price for a particular company will grow at a rate of $5 per week with a standard deviation of $1. An investor believes the stock won’t grow as quickly. The changes in stock price is recorded for ten weeks and are as follows: $4, $3, $2, $3, $1, $7, $2, $1, $1, $2. Perform a hypothesis test using a 5% level of significance. State the null and alternative hypotheses, find the p -value, state your conclusion, and identify the Type I and Type II errors.

• \(H_{0}: \mu = 5\)
• \(H_{a}: \mu < 5\)
• \(p = 0.0082\)

Because \(p < \alpha\), we reject the null hypothesis. There is sufficient evidence to suggest that the stock price of the company grows at a rate less than $5 a week.

• Type I Error: To conclude that the stock price is growing slower than $5 a week when, in fact, the stock price is growing at $5 a week (reject the null hypothesis when the null hypothesis is true).
• Type II Error: To conclude that the stock price is growing at a rate of $5 a week when, in fact, the stock price is growing slower than $5 a week (do not reject the null hypothesis when the null hypothesis is false).

Example \(\PageIndex{5}\)

The National Institute of Standards and Technology provides exact data on conductivity properties of materials. Following are conductivity measurements for 11 randomly selected pieces of a particular type of glass.

1.11; 1.07; 1.11; 1.07; 1.12; 1.08; .98; .98 1.02; .95; .95

Is there convincing evidence that the average conductivity of this type of glass is greater than one? Use a significance level of 0.05. Assume the population is normal.

Let’s follow a four-step process to answer this statistical question.

• \(H_{0}: \mu \leq 1\)
• \(H_{a}: \mu > 1\)
• Plan : We are testing a sample mean without a known population standard deviation. Therefore, we need to use a Student's-t distribution. Assume the underlying population is normal.
• Do the calculations : \(p\text{-value} ( = 0.036)\)

4. State the Conclusions : Since the \(p\text{-value} (= 0.036)\) is less than our alpha value, we will reject the null hypothesis. It is reasonable to state that the data supports the claim that the average conductivity level is greater than one.

The hypothesis test itself has an established process. This can be summarized as follows:

• Determine \(H_{0}\) and \(H_{a}\). Remember, they are contradictory.
• Determine the random variable.
• Determine the distribution for the test.
• Draw a graph, calculate the test statistic, and use the test statistic to calculate the \(p\text{-value}\). (A t -score is an example of test statistics.)
• Compare the preconceived α with the p -value, make a decision (reject or do not reject H 0 ), and write a clear conclusion using English sentences.

Notice that in performing the hypothesis test, you use \(\alpha\) and not \(\beta\). \(\beta\) is needed to help determine the sample size of the data that is used in calculating the \(p\text{-value}\). Remember that the quantity \(1 – \beta\) is called the Power of the Test . A high power is desirable. If the power is too low, statisticians typically increase the sample size while keeping α the same.If the power is low, the null hypothesis might not be rejected when it should be.

• Data from Amit Schitai. Director of Instructional Technology and Distance Learning. LBCC.
• Data from Bloomberg Businessweek . Available online at www.businessweek.com/news/2011- 09-15/nyc-smoking-rate-falls-to-record-low-of-14-bloomberg-says.html.
• Data from energy.gov. Available online at http://energy.gov (accessed June 27. 2013).
• Data from Gallup®. Available online at www.gallup.com (accessed June 27, 2013).
• Data from Growing by Degrees by Allen and Seaman.
• Data from La Leche League International. Available online at www.lalecheleague.org/Law/BAFeb01.html.
• Data from the American Automobile Association. Available online at www.aaa.com (accessed June 27, 2013).
• Data from the American Library Association. Available online at www.ala.org (accessed June 27, 2013).
• Data from the Bureau of Labor Statistics. Available online at http://www.bls.gov/oes/current/oes291111.htm .
• Data from the Centers for Disease Control and Prevention. Available online at www.cdc.gov (accessed June 27, 2013)
• Data from the U.S. Census Bureau, available online at quickfacts.census.gov/qfd/states/00000.html (accessed June 27, 2013).
• Data from the United States Census Bureau. Available online at www.census.gov/hhes/socdemo/language/.
• Data from Toastmasters International. Available online at http://toastmasters.org/artisan/deta...eID=429&Page=1 .
• Data from Weather Underground. Available online at www.wunderground.com (accessed June 27, 2013).
• Federal Bureau of Investigations. “Uniform Crime Reports and Index of Crime in Daviess in the State of Kentucky enforced by Daviess County from 1985 to 2005.” Available online at http://www.disastercenter.com/kentucky/crime/3868.htm (accessed June 27, 2013).
• “Foothill-De Anza Community College District.” De Anza College, Winter 2006. Available online at research.fhda.edu/factbook/DA...t_da_2006w.pdf.
• Johansen, C., J. Boice, Jr., J. McLaughlin, J. Olsen. “Cellular Telephones and Cancer—a Nationwide Cohort Study in Denmark.” Institute of Cancer Epidemiology and the Danish Cancer Society, 93(3):203-7. Available online at http://www.ncbi.nlm.nih.gov/pubmed/11158188 (accessed June 27, 2013).
• Rape, Abuse & Incest National Network. “How often does sexual assault occur?” RAINN, 2009. Available online at www.rainn.org/get-information...sexual-assault (accessed June 27, 2013).

Statistics Tutorial

Descriptive statistics, inferential statistics, stat reference, statistics - hypothesis testing a mean.

A population mean is an average of value a population.

Hypothesis tests are used to check a claim about the size of that population mean.

Hypothesis Testing a Mean

The following steps are used for a hypothesis test:

• Check the conditions
• Define the claims
• Decide the significance level
• Calculate the test statistic

For example:

• Population : Nobel Prize winners
• Category : Age when they received the prize.

And we want to check the claim:

"The average age of Nobel Prize winners when they received the prize is more than 55"

By taking a sample of 30 randomly selected Nobel Prize winners we could find that:

The mean age in the sample (\(\bar{x}\)) is 62.1

The standard deviation of age in the sample (\(s\)) is 13.46

From this sample data we check the claim with the steps below.

1. Checking the Conditions

The conditions for calculating a confidence interval for a proportion are:

• The sample is randomly selected
• The population data is normally distributed
• Sample size is large enough

A moderately large sample size, like 30, is typically large enough.

In the example, the sample size was 30 and it was randomly selected, so the conditions are fulfilled.

Note: Checking if the data is normally distributed can be done with specialized statistical tests.

2. Defining the Claims

We need to define a null hypothesis (\(H_{0}\)) and an alternative hypothesis (\(H_{1}\)) based on the claim we are checking.

The claim was:

In this case, the parameter is the mean age of Nobel Prize winners when they received the prize (\(\mu\)).

The null and alternative hypothesis are then:

Null hypothesis : The average age was 55.

Alternative hypothesis : The average age was more than 55.

Which can be expressed with symbols as:

\(H_{0}\): \(\mu = 55 \)

\(H_{1}\): \(\mu > 55 \)

This is a ' right tailed' test, because the alternative hypothesis claims that the proportion is more than in the null hypothesis.

If the data supports the alternative hypothesis, we reject the null hypothesis and accept the alternative hypothesis.

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3. Deciding the Significance Level

The significance level (\(\alpha\)) is the uncertainty we accept when rejecting the null hypothesis in a hypothesis test.

The significance level is a percentage probability of accidentally making the wrong conclusion.

Typical significance levels are:

• \(\alpha = 0.1\) (10%)
• \(\alpha = 0.05\) (5%)
• \(\alpha = 0.01\) (1%)

A lower significance level means that the evidence in the data needs to be stronger to reject the null hypothesis.

There is no "correct" significance level - it only states the uncertainty of the conclusion.

Note: A 5% significance level means that when we reject a null hypothesis:

We expect to reject a true null hypothesis 5 out of 100 times.

4. Calculating the Test Statistic

The test statistic is used to decide the outcome of the hypothesis test.

The test statistic is a standardized value calculated from the sample.

The formula for the test statistic (TS) of a population mean is:

\(\displaystyle \frac{\bar{x} - \mu}{s} \cdot \sqrt{n} \)

\(\bar{x}-\mu\) is the difference between the sample mean (\(\bar{x}\)) and the claimed population mean (\(\mu\)).

\(s\) is the sample standard deviation .

\(n\) is the sample size.

In our example:

The claimed (\(H_{0}\)) population mean (\(\mu\)) was \( 55 \)

The sample mean (\(\bar{x}\)) was \(62.1\)

The sample standard deviation (\(s\)) was \(13.46\)

The sample size (\(n\)) was \(30\)

So the test statistic (TS) is then:

\(\displaystyle \frac{62.1-55}{13.46} \cdot \sqrt{30} = \frac{7.1}{13.46} \cdot \sqrt{30} \approx 0.528 \cdot 5.477 = \underline{2.889}\)

You can also calculate the test statistic using programming language functions:

With Python use the scipy and math libraries to calculate the test statistic.

With R use built-in math and statistics functions to calculate the test statistic.

5. Concluding

There are two main approaches for making the conclusion of a hypothesis test:

• The critical value approach compares the test statistic with the critical value of the significance level.
• The P-value approach compares the P-value of the test statistic and with the significance level.

Note: The two approaches are only different in how they present the conclusion.

The Critical Value Approach

For the critical value approach we need to find the critical value (CV) of the significance level (\(\alpha\)).

For a population mean test, the critical value (CV) is a T-value from a student's t-distribution .

This critical T-value (CV) defines the rejection region for the test.

The rejection region is an area of probability in the tails of the standard normal distribution.

Because the claim is that the population mean is more than 55, the rejection region is in the right tail:

The student's t-distribution is adjusted for the uncertainty from smaller samples.

This adjustment is called degrees of freedom (df), which is the sample size \((n) - 1\)

In this case the degrees of freedom (df) is: \(30 - 1 = \underline{29} \)

Choosing a significance level (\(\alpha\)) of 0.01, or 1%, we can find the critical T-value from a T-table , or with a programming language function:

With Python use the Scipy Stats library t.ppf() function find the T-Value for an \(\alpha\) = 0.01 at 29 degrees of freedom (df).

With R use the built-in qt() function to find the t-value for an \(\alpha\) = 0.01 at 29 degrees of freedom (df).

Using either method we can find that the critical T-Value is \(\approx \underline{2.462}\)

For a right tailed test we need to check if the test statistic (TS) is bigger than the critical value (CV).

If the test statistic is bigger than the critical value, the test statistic is in the rejection region .

When the test statistic is in the rejection region, we reject the null hypothesis (\(H_{0}\)).

Here, the test statistic (TS) was \(\approx \underline{2.889}\) and the critical value was \(\approx \underline{2.462}\)

Here is an illustration of this test in a graph:

Since the test statistic was bigger than the critical value we reject the null hypothesis.

This means that the sample data supports the alternative hypothesis.

And we can summarize the conclusion stating:

The sample data supports the claim that "The average age of Nobel Prize winners when they received the prize is more than 55" at a 1% significance level .

The P-Value Approach

For the P-value approach we need to find the P-value of the test statistic (TS).

If the P-value is smaller than the significance level (\(\alpha\)), we reject the null hypothesis (\(H_{0}\)).

The test statistic was found to be \( \approx \underline{2.889} \)

For a population proportion test, the test statistic is a T-Value from a student's t-distribution .

Because this is a right tailed test, we need to find the P-value of a t-value bigger than 2.889.

The student's t-distribution is adjusted according to degrees of freedom (df), which is the sample size \((30) - 1 = \underline{29}\)

We can find the P-value using a T-table , or with a programming language function:

With Python use the Scipy Stats library t.cdf() function find the P-value of a T-value bigger than 2.889 at 29 degrees of freedom (df):

With R use the built-in pt() function find the P-value of a T-Value bigger than 2.889 at 29 degrees of freedom (df):

Using either method we can find that the P-value is \(\approx \underline{0.0036}\)

This tells us that the significance level (\(\alpha\)) would need to be bigger than 0.0036, or 0.36%, to reject the null hypothesis.

This P-value is smaller than any of the common significance levels (10%, 5%, 1%).

So the null hypothesis is rejected at all of these significance levels.

The sample data supports the claim that "The average age of Nobel Prize winners when they received the prize is more than 55" at a 10%, 5%, or 1% significance level .

Note: An outcome of an hypothesis test that rejects the null hypothesis with a p-value of 0.36% means:

For this p-value, we only expect to reject a true null hypothesis 36 out of 10000 times.

Calculating a P-Value for a Hypothesis Test with Programming

Many programming languages can calculate the P-value to decide outcome of a hypothesis test.

Using software and programming to calculate statistics is more common for bigger sets of data, as calculating manually becomes difficult.

The P-value calculated here will tell us the lowest possible significance level where the null-hypothesis can be rejected.

With Python use the scipy and math libraries to calculate the P-value for a right tailed hypothesis test for a mean.

Here, the sample size is 30, the sample mean is 62.1, the sample standard deviation is 13.46, and the test is for a mean bigger than 55.

With R use built-in math and statistics functions find the P-value for a right tailed hypothesis test for a mean.

Left-Tailed and Two-Tailed Tests

This was an example of a right tailed test, where the alternative hypothesis claimed that parameter is bigger than the null hypothesis claim.

You can check out an equivalent step-by-step guide for other types here:

• Left-Tailed Test
• Two-Tailed Test

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Unit 12: Significance tests (hypothesis testing)

About this unit.

Significance tests give us a formal process for using sample data to evaluate the likelihood of some claim about a population value. Learn how to conduct significance tests and calculate p-values to see how likely a sample result is to occur by random chance. You'll also see how we use p-values to make conclusions about hypotheses.

The idea of significance tests

• Simple hypothesis testing (Opens a modal)
• Idea behind hypothesis testing (Opens a modal)
• Examples of null and alternative hypotheses (Opens a modal)
• P-values and significance tests (Opens a modal)
• Comparing P-values to different significance levels (Opens a modal)
• Estimating a P-value from a simulation (Opens a modal)
• Using P-values to make conclusions (Opens a modal)
• Simple hypothesis testing Get 3 of 4 questions to level up!
• Writing null and alternative hypotheses Get 3 of 4 questions to level up!
• Estimating P-values from simulations Get 3 of 4 questions to level up!

Error probabilities and power

• Introduction to Type I and Type II errors (Opens a modal)
• Type 1 errors (Opens a modal)
• Examples identifying Type I and Type II errors (Opens a modal)
• Introduction to power in significance tests (Opens a modal)
• Examples thinking about power in significance tests (Opens a modal)
• Consequences of errors and significance (Opens a modal)
• Type I vs Type II error Get 3 of 4 questions to level up!
• Error probabilities and power Get 3 of 4 questions to level up!

Tests about a population proportion

• Constructing hypotheses for a significance test about a proportion (Opens a modal)
• Conditions for a z test about a proportion (Opens a modal)
• Reference: Conditions for inference on a proportion (Opens a modal)
• Calculating a z statistic in a test about a proportion (Opens a modal)
• Calculating a P-value given a z statistic (Opens a modal)
• Making conclusions in a test about a proportion (Opens a modal)
• Writing hypotheses for a test about a proportion Get 3 of 4 questions to level up!
• Conditions for a z test about a proportion Get 3 of 4 questions to level up!
• Calculating the test statistic in a z test for a proportion Get 3 of 4 questions to level up!
• Calculating the P-value in a z test for a proportion Get 3 of 4 questions to level up!
• Making conclusions in a z test for a proportion Get 3 of 4 questions to level up!

Tests about a population mean

• Writing hypotheses for a significance test about a mean (Opens a modal)
• Conditions for a t test about a mean (Opens a modal)
• Reference: Conditions for inference on a mean (Opens a modal)
• When to use z or t statistics in significance tests (Opens a modal)
• Example calculating t statistic for a test about a mean (Opens a modal)
• Using TI calculator for P-value from t statistic (Opens a modal)
• Using a table to estimate P-value from t statistic (Opens a modal)
• Comparing P-value from t statistic to significance level (Opens a modal)
• Free response example: Significance test for a mean (Opens a modal)
• Writing hypotheses for a test about a mean Get 3 of 4 questions to level up!
• Conditions for a t test about a mean Get 3 of 4 questions to level up!
• Calculating the test statistic in a t test for a mean Get 3 of 4 questions to level up!
• Calculating the P-value in a t test for a mean Get 3 of 4 questions to level up!
• Making conclusions in a t test for a mean Get 3 of 4 questions to level up!

More significance testing videos

• Hypothesis testing and p-values (Opens a modal)
• One-tailed and two-tailed tests (Opens a modal)
• Z-statistics vs. T-statistics (Opens a modal)
• Small sample hypothesis test (Opens a modal)
• Large sample proportion hypothesis testing (Opens a modal)

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S.3.2 hypothesis testing (p-value approach).

The P -value approach involves determining "likely" or "unlikely" by determining the probability — assuming the null hypothesis was true — of observing a more extreme test statistic in the direction of the alternative hypothesis than the one observed. If the P -value is small, say less than (or equal to) \(\alpha\), then it is "unlikely." And, if the P -value is large, say more than \(\alpha\), then it is "likely."

If the P -value is less than (or equal to) \(\alpha\), then the null hypothesis is rejected in favor of the alternative hypothesis. And, if the P -value is greater than \(\alpha\), then the null hypothesis is not rejected.

Specifically, the four steps involved in using the P -value approach to conducting any hypothesis test are:

• Specify the null and alternative hypotheses.
• Using the sample data and assuming the null hypothesis is true, calculate the value of the test statistic. Again, to conduct the hypothesis test for the population mean μ , we use the t -statistic \(t^*=\frac{\bar{x}-\mu}{s/\sqrt{n}}\) which follows a t -distribution with n - 1 degrees of freedom.
• Using the known distribution of the test statistic, calculate the P -value : "If the null hypothesis is true, what is the probability that we'd observe a more extreme test statistic in the direction of the alternative hypothesis than we did?" (Note how this question is equivalent to the question answered in criminal trials: "If the defendant is innocent, what is the chance that we'd observe such extreme criminal evidence?")
• Set the significance level, \(\alpha\), the probability of making a Type I error to be small — 0.01, 0.05, or 0.10. Compare the P -value to \(\alpha\). If the P -value is less than (or equal to) \(\alpha\), reject the null hypothesis in favor of the alternative hypothesis. If the P -value is greater than \(\alpha\), do not reject the null hypothesis.

Example S.3.2.1

Mean gpa section  .

In our example concerning the mean grade point average, suppose that our random sample of n = 15 students majoring in mathematics yields a test statistic t * equaling 2.5. Since n = 15, our test statistic t * has n - 1 = 14 degrees of freedom. Also, suppose we set our significance level α at 0.05 so that we have only a 5% chance of making a Type I error.

Right Tailed

The P -value for conducting the right-tailed test H 0 : μ = 3 versus H A : μ > 3 is the probability that we would observe a test statistic greater than t * = 2.5 if the population mean \(\mu\) really were 3. Recall that probability equals the area under the probability curve. The P -value is therefore the area under a t n - 1 = t 14 curve and to the right of the test statistic t * = 2.5. It can be shown using statistical software that the P -value is 0.0127. The graph depicts this visually.

The P -value, 0.0127, tells us it is "unlikely" that we would observe such an extreme test statistic t * in the direction of H A if the null hypothesis were true. Therefore, our initial assumption that the null hypothesis is true must be incorrect. That is, since the P -value, 0.0127, is less than \(\alpha\) = 0.05, we reject the null hypothesis H 0 : μ = 3 in favor of the alternative hypothesis H A : μ > 3.

Note that we would not reject H 0 : μ = 3 in favor of H A : μ > 3 if we lowered our willingness to make a Type I error to \(\alpha\) = 0.01 instead, as the P -value, 0.0127, is then greater than \(\alpha\) = 0.01.

Left Tailed

In our example concerning the mean grade point average, suppose that our random sample of n = 15 students majoring in mathematics yields a test statistic t * instead of equaling -2.5. The P -value for conducting the left-tailed test H 0 : μ = 3 versus H A : μ < 3 is the probability that we would observe a test statistic less than t * = -2.5 if the population mean μ really were 3. The P -value is therefore the area under a t n - 1 = t 14 curve and to the left of the test statistic t* = -2.5. It can be shown using statistical software that the P -value is 0.0127. The graph depicts this visually.

The P -value, 0.0127, tells us it is "unlikely" that we would observe such an extreme test statistic t * in the direction of H A if the null hypothesis were true. Therefore, our initial assumption that the null hypothesis is true must be incorrect. That is, since the P -value, 0.0127, is less than α = 0.05, we reject the null hypothesis H 0 : μ = 3 in favor of the alternative hypothesis H A : μ < 3.

Note that we would not reject H 0 : μ = 3 in favor of H A : μ < 3 if we lowered our willingness to make a Type I error to α = 0.01 instead, as the P -value, 0.0127, is then greater than \(\alpha\) = 0.01.

In our example concerning the mean grade point average, suppose again that our random sample of n = 15 students majoring in mathematics yields a test statistic t * instead of equaling -2.5. The P -value for conducting the two-tailed test H 0 : μ = 3 versus H A : μ ≠ 3 is the probability that we would observe a test statistic less than -2.5 or greater than 2.5 if the population mean μ really was 3. That is, the two-tailed test requires taking into account the possibility that the test statistic could fall into either tail (hence the name "two-tailed" test). The P -value is, therefore, the area under a t n - 1 = t 14 curve to the left of -2.5 and to the right of 2.5. It can be shown using statistical software that the P -value is 0.0127 + 0.0127, or 0.0254. The graph depicts this visually.

Note that the P -value for a two-tailed test is always two times the P -value for either of the one-tailed tests. The P -value, 0.0254, tells us it is "unlikely" that we would observe such an extreme test statistic t * in the direction of H A if the null hypothesis were true. Therefore, our initial assumption that the null hypothesis is true must be incorrect. That is, since the P -value, 0.0254, is less than α = 0.05, we reject the null hypothesis H 0 : μ = 3 in favor of the alternative hypothesis H A : μ ≠ 3.

Note that we would not reject H 0 : μ = 3 in favor of H A : μ ≠ 3 if we lowered our willingness to make a Type I error to α = 0.01 instead, as the P -value, 0.0254, is then greater than \(\alpha\) = 0.01.

Now that we have reviewed the critical value and P -value approach procedures for each of the three possible hypotheses, let's look at three new examples — one of a right-tailed test, one of a left-tailed test, and one of a two-tailed test.

The good news is that, whenever possible, we will take advantage of the test statistics and P -values reported in statistical software, such as Minitab, to conduct our hypothesis tests in this course.

Tutorial Playlist

Statistics tutorial, everything you need to know about the probability density function in statistics, the best guide to understand central limit theorem, an in-depth guide to measures of central tendency : mean, median and mode, the ultimate guide to understand conditional probability.

A Comprehensive Look at Percentile in Statistics

The Best Guide to Understand Bayes Theorem

Everything you need to know about the normal distribution, an in-depth explanation of cumulative distribution function, a complete guide to chi-square test, what is hypothesis testing in statistics types and examples, understanding the fundamentals of arithmetic and geometric progression, the definitive guide to understand spearman’s rank correlation, a comprehensive guide to understand mean squared error, all you need to know about the empirical rule in statistics, the complete guide to skewness and kurtosis, a holistic look at bernoulli distribution.

All You Need to Know About Bias in Statistics

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The Complete Guide to Understand Pearson's Correlation

A complete guide on the types of statistical studies, everything you need to know about poisson distribution, your best guide to understand correlation vs. regression, the most comprehensive guide for beginners on what is correlation.

Lesson 10 of 24 By Avijeet Biswal

Table of Contents

In today’s data-driven world , decisions are based on data all the time. Hypothesis plays a crucial role in that process, whether it may be making business decisions, in the health sector, academia, or in quality improvement. Without hypothesis & hypothesis tests, you risk drawing the wrong conclusions and making bad decisions. In this tutorial, you will look at Hypothesis Testing in Statistics.

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What Is Hypothesis Testing in Statistics?

Hypothesis Testing is a type of statistical analysis in which you put your assumptions about a population parameter to the test. It is used to estimate the relationship between 2 statistical variables.

Let's discuss few examples of statistical hypothesis from real-life - 

• A teacher assumes that 60% of his college's students come from lower-middle-class families.
• A doctor believes that 3D (Diet, Dose, and Discipline) is 90% effective for diabetic patients.

Now that you know about hypothesis testing, look at the two types of hypothesis testing in statistics.

Hypothesis Testing Formula

Z = ( x̅ – μ0 ) / (σ /√n)

• Here, x̅ is the sample mean,
• μ0 is the population mean,
• σ is the standard deviation,
• n is the sample size.

How Hypothesis Testing Works?

An analyst performs hypothesis testing on a statistical sample to present evidence of the plausibility of the null hypothesis. Measurements and analyses are conducted on a random sample of the population to test a theory. Analysts use a random population sample to test two hypotheses: the null and alternative hypotheses.

The null hypothesis is typically an equality hypothesis between population parameters; for example, a null hypothesis may claim that the population means return equals zero. The alternate hypothesis is essentially the inverse of the null hypothesis (e.g., the population means the return is not equal to zero). As a result, they are mutually exclusive, and only one can be correct. One of the two possibilities, however, will always be correct.

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Null Hypothesis and Alternate Hypothesis

The Null Hypothesis is the assumption that the event will not occur. A null hypothesis has no bearing on the study's outcome unless it is rejected.

H0 is the symbol for it, and it is pronounced H-naught.

The Alternate Hypothesis is the logical opposite of the null hypothesis. The acceptance of the alternative hypothesis follows the rejection of the null hypothesis. H1 is the symbol for it.

Let's understand this with an example.

A sanitizer manufacturer claims that its product kills 95 percent of germs on average. 

To put this company's claim to the test, create a null and alternate hypothesis.

H0 (Null Hypothesis): Average = 95%.

Alternative Hypothesis (H1): The average is less than 95%.

Another straightforward example to understand this concept is determining whether or not a coin is fair and balanced. The null hypothesis states that the probability of a show of heads is equal to the likelihood of a show of tails. In contrast, the alternate theory states that the probability of a show of heads and tails would be very different.

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Hypothesis Testing Calculation With Examples

Let's consider a hypothesis test for the average height of women in the United States. Suppose our null hypothesis is that the average height is 5'4". We gather a sample of 100 women and determine that their average height is 5'5". The standard deviation of population is 2.

To calculate the z-score, we would use the following formula:

z = ( x̅ – μ0 ) / (σ /√n)

z = (5'5" - 5'4") / (2" / √100)

z = 0.5 / (0.045)

We will reject the null hypothesis as the z-score of 11.11 is very large and conclude that there is evidence to suggest that the average height of women in the US is greater than 5'4".

Steps of Hypothesis Testing

Hypothesis testing is a statistical method to determine if there is enough evidence in a sample of data to infer that a certain condition is true for the entire population. Here’s a breakdown of the typical steps involved in hypothesis testing:

Formulate Hypotheses

• Null Hypothesis (H0): This hypothesis states that there is no effect or difference, and it is the hypothesis you attempt to reject with your test.
• Alternative Hypothesis (H1 or Ha): This hypothesis is what you might believe to be true or hope to prove true. It is usually considered the opposite of the null hypothesis.

Choose the Significance Level (α)

The significance level, often denoted by alpha (α), is the probability of rejecting the null hypothesis when it is true. Common choices for α are 0.05 (5%), 0.01 (1%), and 0.10 (10%).

Select the Appropriate Test

Choose a statistical test based on the type of data and the hypothesis. Common tests include t-tests, chi-square tests, ANOVA, and regression analysis . The selection depends on data type, distribution, sample size, and whether the hypothesis is one-tailed or two-tailed.

Collect Data

Gather the data that will be analyzed in the test. This data should be representative of the population to infer conclusions accurately.

Calculate the Test Statistic

Based on the collected data and the chosen test, calculate a test statistic that reflects how much the observed data deviates from the null hypothesis.

Determine the p-value

The p-value is the probability of observing test results at least as extreme as the results observed, assuming the null hypothesis is correct. It helps determine the strength of the evidence against the null hypothesis.

Make a Decision

Compare the p-value to the chosen significance level:

• If the p-value ≤ α: Reject the null hypothesis, suggesting sufficient evidence in the data supports the alternative hypothesis.
• If the p-value > α: Do not reject the null hypothesis, suggesting insufficient evidence to support the alternative hypothesis.

Report the Results

Present the findings from the hypothesis test, including the test statistic, p-value, and the conclusion about the hypotheses.

Perform Post-hoc Analysis (if necessary)

Depending on the results and the study design, further analysis may be needed to explore the data more deeply or to address multiple comparisons if several hypotheses were tested simultaneously.

Types of Hypothesis Testing

To determine whether a discovery or relationship is statistically significant, hypothesis testing uses a z-test. It usually checks to see if two means are the same (the null hypothesis). Only when the population standard deviation is known and the sample size is 30 data points or more, can a z-test be applied.

A statistical test called a t-test is employed to compare the means of two groups. To determine whether two groups differ or if a procedure or treatment affects the population of interest, it is frequently used in hypothesis testing.

Chi-Square 

You utilize a Chi-square test for hypothesis testing concerning whether your data is as predicted. To determine if the expected and observed results are well-fitted, the Chi-square test analyzes the differences between categorical variables from a random sample. The test's fundamental premise is that the observed values in your data should be compared to the predicted values that would be present if the null hypothesis were true.

Hypothesis Testing and Confidence Intervals

Both confidence intervals and hypothesis tests are inferential techniques that depend on approximating the sample distribution. Data from a sample is used to estimate a population parameter using confidence intervals. Data from a sample is used in hypothesis testing to examine a given hypothesis. We must have a postulated parameter to conduct hypothesis testing.

Bootstrap distributions and randomization distributions are created using comparable simulation techniques. The observed sample statistic is the focal point of a bootstrap distribution, whereas the null hypothesis value is the focal point of a randomization distribution.

A variety of feasible population parameter estimates are included in confidence ranges. In this lesson, we created just two-tailed confidence intervals. There is a direct connection between these two-tail confidence intervals and these two-tail hypothesis tests. The results of a two-tailed hypothesis test and two-tailed confidence intervals typically provide the same results. In other words, a hypothesis test at the 0.05 level will virtually always fail to reject the null hypothesis if the 95% confidence interval contains the predicted value. A hypothesis test at the 0.05 level will nearly certainly reject the null hypothesis if the 95% confidence interval does not include the hypothesized parameter.

Simple and Composite Hypothesis Testing

Depending on the population distribution, you can classify the statistical hypothesis into two types.

Simple Hypothesis: A simple hypothesis specifies an exact value for the parameter.

Composite Hypothesis: A composite hypothesis specifies a range of values.

A company is claiming that their average sales for this quarter are 1000 units. This is an example of a simple hypothesis.

Suppose the company claims that the sales are in the range of 900 to 1000 units. Then this is a case of a composite hypothesis.

One-Tailed and Two-Tailed Hypothesis Testing

The One-Tailed test, also called a directional test, considers a critical region of data that would result in the null hypothesis being rejected if the test sample falls into it, inevitably meaning the acceptance of the alternate hypothesis.

In a one-tailed test, the critical distribution area is one-sided, meaning the test sample is either greater or lesser than a specific value.

In two tails, the test sample is checked to be greater or less than a range of values in a Two-Tailed test, implying that the critical distribution area is two-sided.

If the sample falls within this range, the alternate hypothesis will be accepted, and the null hypothesis will be rejected.

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Right Tailed Hypothesis Testing

If the larger than (>) sign appears in your hypothesis statement, you are using a right-tailed test, also known as an upper test. Or, to put it another way, the disparity is to the right. For instance, you can contrast the battery life before and after a change in production. Your hypothesis statements can be the following if you want to know if the battery life is longer than the original (let's say 90 hours):

• The null hypothesis is (H0 <= 90) or less change.
• A possibility is that battery life has risen (H1) > 90.

The crucial point in this situation is that the alternate hypothesis (H1), not the null hypothesis, decides whether you get a right-tailed test.

Left Tailed Hypothesis Testing

Alternative hypotheses that assert the true value of a parameter is lower than the null hypothesis are tested with a left-tailed test; they are indicated by the asterisk "<".

Suppose H0: mean = 50 and H1: mean not equal to 50

According to the H1, the mean can be greater than or less than 50. This is an example of a Two-tailed test.

In a similar manner, if H0: mean >=50, then H1: mean <50

Here the mean is less than 50. It is called a One-tailed test.

Type 1 and Type 2 Error

A hypothesis test can result in two types of errors.

Type 1 Error: A Type-I error occurs when sample results reject the null hypothesis despite being true.

Type 2 Error: A Type-II error occurs when the null hypothesis is not rejected when it is false, unlike a Type-I error.

Suppose a teacher evaluates the examination paper to decide whether a student passes or fails.

H0: Student has passed

H1: Student has failed

Type I error will be the teacher failing the student [rejects H0] although the student scored the passing marks [H0 was true]. 

Type II error will be the case where the teacher passes the student [do not reject H0] although the student did not score the passing marks [H1 is true].

Level of Significance

The alpha value is a criterion for determining whether a test statistic is statistically significant. In a statistical test, Alpha represents an acceptable probability of a Type I error. Because alpha is a probability, it can be anywhere between 0 and 1. In practice, the most commonly used alpha values are 0.01, 0.05, and 0.1, which represent a 1%, 5%, and 10% chance of a Type I error, respectively (i.e. rejecting the null hypothesis when it is in fact correct).

A p-value is a metric that expresses the likelihood that an observed difference could have occurred by chance. As the p-value decreases the statistical significance of the observed difference increases. If the p-value is too low, you reject the null hypothesis.

Here you have taken an example in which you are trying to test whether the new advertising campaign has increased the product's sales. The p-value is the likelihood that the null hypothesis, which states that there is no change in the sales due to the new advertising campaign, is true. If the p-value is .30, then there is a 30% chance that there is no increase or decrease in the product's sales.  If the p-value is 0.03, then there is a 3% probability that there is no increase or decrease in the sales value due to the new advertising campaign. As you can see, the lower the p-value, the chances of the alternate hypothesis being true increases, which means that the new advertising campaign causes an increase or decrease in sales.

Why Is Hypothesis Testing Important in Research Methodology?

Hypothesis testing is crucial in research methodology for several reasons:

• Provides evidence-based conclusions: It allows researchers to make objective conclusions based on empirical data, providing evidence to support or refute their research hypotheses.
• Supports decision-making: It helps make informed decisions, such as accepting or rejecting a new treatment, implementing policy changes, or adopting new practices.
• Adds rigor and validity: It adds scientific rigor to research using statistical methods to analyze data, ensuring that conclusions are based on sound statistical evidence.
• Contributes to the advancement of knowledge: By testing hypotheses, researchers contribute to the growth of knowledge in their respective fields by confirming existing theories or discovering new patterns and relationships.

When Did Hypothesis Testing Begin?

Hypothesis testing as a formalized process began in the early 20th century, primarily through the work of statisticians such as Ronald A. Fisher, Jerzy Neyman, and Egon Pearson. The development of hypothesis testing is closely tied to the evolution of statistical methods during this period.

• Ronald A. Fisher (1920s): Fisher was one of the key figures in developing the foundation for modern statistical science. In the 1920s, he introduced the concept of the null hypothesis in his book "Statistical Methods for Research Workers" (1925). Fisher also developed significance testing to examine the likelihood of observing the collected data if the null hypothesis were true. He introduced p-values to determine the significance of the observed results.
• Neyman-Pearson Framework (1930s): Jerzy Neyman and Egon Pearson built on Fisher’s work and formalized the process of hypothesis testing even further. In the 1930s, they introduced the concepts of Type I and Type II errors and developed a decision-making framework widely used in hypothesis testing today. Their approach emphasized the balance between these errors and introduced the concepts of the power of a test and the alternative hypothesis.

The dialogue between Fisher's and Neyman-Pearson's approaches shaped the methods and philosophy of statistical hypothesis testing used today. Fisher emphasized the evidential interpretation of the p-value. At the same time, Neyman and Pearson advocated for a decision-theoretical approach in which hypotheses are either accepted or rejected based on pre-determined significance levels and power considerations.

The application and methodology of hypothesis testing have since become a cornerstone of statistical analysis across various scientific disciplines, marking a significant statistical development.

Limitations of Hypothesis Testing

Hypothesis testing has some limitations that researchers should be aware of:

• It cannot prove or establish the truth: Hypothesis testing provides evidence to support or reject a hypothesis, but it cannot confirm the absolute truth of the research question.
• Results are sample-specific: Hypothesis testing is based on analyzing a sample from a population, and the conclusions drawn are specific to that particular sample.
• Possible errors: During hypothesis testing, there is a chance of committing type I error (rejecting a true null hypothesis) or type II error (failing to reject a false null hypothesis).
• Assumptions and requirements: Different tests have specific assumptions and requirements that must be met to accurately interpret results.

After reading this tutorial, you would have a much better understanding of hypothesis testing, one of the most important concepts in the field of Data Science . The majority of hypotheses are based on speculation about observed behavior, natural phenomena, or established theories.

If you are interested in statistics of data science and skills needed for such a career, you ought to explore the Post Graduate Program in Data Science.

If you have any questions regarding this ‘Hypothesis Testing In Statistics’ tutorial, do share them in the comment section. Our subject matter expert will respond to your queries. Happy learning!

1. What is hypothesis testing in statistics with example?

Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence. An example: testing if a new drug improves patient recovery (Ha) compared to the standard treatment (H0) based on collected patient data.

2. What is H0 and H1 in statistics?

In statistics, H0​ and H1​ represent the null and alternative hypotheses. The null hypothesis, H0​, is the default assumption that no effect or difference exists between groups or conditions. The alternative hypothesis, H1​, is the competing claim suggesting an effect or a difference. Statistical tests determine whether to reject the null hypothesis in favor of the alternative hypothesis based on the data.

3. What is a simple hypothesis with an example?

A simple hypothesis is a specific statement predicting a single relationship between two variables. It posits a direct and uncomplicated outcome. For example, a simple hypothesis might state, "Increased sunlight exposure increases the growth rate of sunflowers." Here, the hypothesis suggests a direct relationship between the amount of sunlight (independent variable) and the growth rate of sunflowers (dependent variable), with no additional variables considered.

4. What are the 2 types of hypothesis testing?

• One-tailed (or one-sided) test: Tests for the significance of an effect in only one direction, either positive or negative.
• Two-tailed (or two-sided) test: Tests for the significance of an effect in both directions, allowing for the possibility of a positive or negative effect.

The choice between one-tailed and two-tailed tests depends on the specific research question and the directionality of the expected effect.

5. What are the 3 major types of hypothesis?

The three major types of hypotheses are:

• Null Hypothesis (H0): Represents the default assumption, stating that there is no significant effect or relationship in the data.
• Alternative Hypothesis (Ha): Contradicts the null hypothesis and proposes a specific effect or relationship that researchers want to investigate.
• Nondirectional Hypothesis: An alternative hypothesis that doesn't specify the direction of the effect, leaving it open for both positive and negative possibilities.

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Hypothesis Testing

Hypothesis testing is a tool for making statistical inferences about the population data. It is an analysis tool that tests assumptions and determines how likely something is within a given standard of accuracy. Hypothesis testing provides a way to verify whether the results of an experiment are valid.

A null hypothesis and an alternative hypothesis are set up before performing the hypothesis testing. This helps to arrive at a conclusion regarding the sample obtained from the population. In this article, we will learn more about hypothesis testing, its types, steps to perform the testing, and associated examples.

What is Hypothesis Testing in Statistics?

Hypothesis testing uses sample data from the population to draw useful conclusions regarding the population probability distribution . It tests an assumption made about the data using different types of hypothesis testing methodologies. The hypothesis testing results in either rejecting or not rejecting the null hypothesis.

Hypothesis Testing Definition

Hypothesis testing can be defined as a statistical tool that is used to identify if the results of an experiment are meaningful or not. It involves setting up a null hypothesis and an alternative hypothesis. These two hypotheses will always be mutually exclusive. This means that if the null hypothesis is true then the alternative hypothesis is false and vice versa. An example of hypothesis testing is setting up a test to check if a new medicine works on a disease in a more efficient manner.

Null Hypothesis

The null hypothesis is a concise mathematical statement that is used to indicate that there is no difference between two possibilities. In other words, there is no difference between certain characteristics of data. This hypothesis assumes that the outcomes of an experiment are based on chance alone. It is denoted as \(H_{0}\). Hypothesis testing is used to conclude if the null hypothesis can be rejected or not. Suppose an experiment is conducted to check if girls are shorter than boys at the age of 5. The null hypothesis will say that they are the same height.

Alternative Hypothesis

The alternative hypothesis is an alternative to the null hypothesis. It is used to show that the observations of an experiment are due to some real effect. It indicates that there is a statistical significance between two possible outcomes and can be denoted as \(H_{1}\) or \(H_{a}\). For the above-mentioned example, the alternative hypothesis would be that girls are shorter than boys at the age of 5.

Hypothesis Testing P Value

In hypothesis testing, the p value is used to indicate whether the results obtained after conducting a test are statistically significant or not. It also indicates the probability of making an error in rejecting or not rejecting the null hypothesis.This value is always a number between 0 and 1. The p value is compared to an alpha level, \(\alpha\) or significance level. The alpha level can be defined as the acceptable risk of incorrectly rejecting the null hypothesis. The alpha level is usually chosen between 1% to 5%.

Hypothesis Testing Critical region

All sets of values that lead to rejecting the null hypothesis lie in the critical region. Furthermore, the value that separates the critical region from the non-critical region is known as the critical value.

Hypothesis Testing Formula

Depending upon the type of data available and the size, different types of hypothesis testing are used to determine whether the null hypothesis can be rejected or not. The hypothesis testing formula for some important test statistics are given below:

• z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\). \(\overline{x}\) is the sample mean, \(\mu\) is the population mean, \(\sigma\) is the population standard deviation and n is the size of the sample.
• t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\). s is the sample standard deviation.
• \(\chi ^{2} = \sum \frac{(O_{i}-E_{i})^{2}}{E_{i}}\). \(O_{i}\) is the observed value and \(E_{i}\) is the expected value.

We will learn more about these test statistics in the upcoming section.

Types of Hypothesis Testing

Selecting the correct test for performing hypothesis testing can be confusing. These tests are used to determine a test statistic on the basis of which the null hypothesis can either be rejected or not rejected. Some of the important tests used for hypothesis testing are given below.

Hypothesis Testing Z Test

A z test is a way of hypothesis testing that is used for a large sample size (n ≥ 30). It is used to determine whether there is a difference between the population mean and the sample mean when the population standard deviation is known. It can also be used to compare the mean of two samples. It is used to compute the z test statistic. The formulas are given as follows:

• One sample: z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).
• Two samples: z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).

Hypothesis Testing t Test

The t test is another method of hypothesis testing that is used for a small sample size (n < 30). It is also used to compare the sample mean and population mean. However, the population standard deviation is not known. Instead, the sample standard deviation is known. The mean of two samples can also be compared using the t test.

• One sample: t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\).
• Two samples: t = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{s_{1}^{2}}{n_{1}}+\frac{s_{2}^{2}}{n_{2}}}}\).

Hypothesis Testing Chi Square

The Chi square test is a hypothesis testing method that is used to check whether the variables in a population are independent or not. It is used when the test statistic is chi-squared distributed.

One Tailed Hypothesis Testing

One tailed hypothesis testing is done when the rejection region is only in one direction. It can also be known as directional hypothesis testing because the effects can be tested in one direction only. This type of testing is further classified into the right tailed test and left tailed test.

Right Tailed Hypothesis Testing

The right tail test is also known as the upper tail test. This test is used to check whether the population parameter is greater than some value. The null and alternative hypotheses for this test are given as follows:

\(H_{0}\): The population parameter is ≤ some value

\(H_{1}\): The population parameter is > some value.

If the test statistic has a greater value than the critical value then the null hypothesis is rejected

Left Tailed Hypothesis Testing

The left tail test is also known as the lower tail test. It is used to check whether the population parameter is less than some value. The hypotheses for this hypothesis testing can be written as follows:

\(H_{0}\): The population parameter is ≥ some value

\(H_{1}\): The population parameter is < some value.

The null hypothesis is rejected if the test statistic has a value lesser than the critical value.

Two Tailed Hypothesis Testing

In this hypothesis testing method, the critical region lies on both sides of the sampling distribution. It is also known as a non - directional hypothesis testing method. The two-tailed test is used when it needs to be determined if the population parameter is assumed to be different than some value. The hypotheses can be set up as follows:

\(H_{0}\): the population parameter = some value

\(H_{1}\): the population parameter ≠ some value

The null hypothesis is rejected if the test statistic has a value that is not equal to the critical value.

Hypothesis Testing Steps

Hypothesis testing can be easily performed in five simple steps. The most important step is to correctly set up the hypotheses and identify the right method for hypothesis testing. The basic steps to perform hypothesis testing are as follows:

• Step 1: Set up the null hypothesis by correctly identifying whether it is the left-tailed, right-tailed, or two-tailed hypothesis testing.
• Step 2: Set up the alternative hypothesis.
• Step 3: Choose the correct significance level, \(\alpha\), and find the critical value.
• Step 4: Calculate the correct test statistic (z, t or \(\chi\)) and p-value.
• Step 5: Compare the test statistic with the critical value or compare the p-value with \(\alpha\) to arrive at a conclusion. In other words, decide if the null hypothesis is to be rejected or not.

Hypothesis Testing Example

The best way to solve a problem on hypothesis testing is by applying the 5 steps mentioned in the previous section. Suppose a researcher claims that the mean average weight of men is greater than 100kgs with a standard deviation of 15kgs. 30 men are chosen with an average weight of 112.5 Kgs. Using hypothesis testing, check if there is enough evidence to support the researcher's claim. The confidence interval is given as 95%.

Step 1: This is an example of a right-tailed test. Set up the null hypothesis as \(H_{0}\): \(\mu\) = 100.

Step 2: The alternative hypothesis is given by \(H_{1}\): \(\mu\) > 100.

Step 3: As this is a one-tailed test, \(\alpha\) = 100% - 95% = 5%. This can be used to determine the critical value.

1 - \(\alpha\) = 1 - 0.05 = 0.95

0.95 gives the required area under the curve. Now using a normal distribution table, the area 0.95 is at z = 1.645. A similar process can be followed for a t-test. The only additional requirement is to calculate the degrees of freedom given by n - 1.

Step 4: Calculate the z test statistic. This is because the sample size is 30. Furthermore, the sample and population means are known along with the standard deviation.

z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).

\(\mu\) = 100, \(\overline{x}\) = 112.5, n = 30, \(\sigma\) = 15

z = \(\frac{112.5-100}{\frac{15}{\sqrt{30}}}\) = 4.56

Step 5: Conclusion. As 4.56 > 1.645 thus, the null hypothesis can be rejected.

Hypothesis Testing and Confidence Intervals

Confidence intervals form an important part of hypothesis testing. This is because the alpha level can be determined from a given confidence interval. Suppose a confidence interval is given as 95%. Subtract the confidence interval from 100%. This gives 100 - 95 = 5% or 0.05. This is the alpha value of a one-tailed hypothesis testing. To obtain the alpha value for a two-tailed hypothesis testing, divide this value by 2. This gives 0.05 / 2 = 0.025.

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Important Notes on Hypothesis Testing

• Hypothesis testing is a technique that is used to verify whether the results of an experiment are statistically significant.
• It involves the setting up of a null hypothesis and an alternate hypothesis.
• There are three types of tests that can be conducted under hypothesis testing - z test, t test, and chi square test.
• Hypothesis testing can be classified as right tail, left tail, and two tail tests.

Examples on Hypothesis Testing

• Example 1: The average weight of a dumbbell in a gym is 90lbs. However, a physical trainer believes that the average weight might be higher. A random sample of 5 dumbbells with an average weight of 110lbs and a standard deviation of 18lbs. Using hypothesis testing check if the physical trainer's claim can be supported for a 95% confidence level. Solution: As the sample size is lesser than 30, the t-test is used. \(H_{0}\): \(\mu\) = 90, \(H_{1}\): \(\mu\) > 90 \(\overline{x}\) = 110, \(\mu\) = 90, n = 5, s = 18. \(\alpha\) = 0.05 Using the t-distribution table, the critical value is 2.132 t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\) t = 2.484 As 2.484 > 2.132, the null hypothesis is rejected. Answer: The average weight of the dumbbells may be greater than 90lbs
• Example 2: The average score on a test is 80 with a standard deviation of 10. With a new teaching curriculum introduced it is believed that this score will change. On random testing, the score of 38 students, the mean was found to be 88. With a 0.05 significance level, is there any evidence to support this claim? Solution: This is an example of two-tail hypothesis testing. The z test will be used. \(H_{0}\): \(\mu\) = 80, \(H_{1}\): \(\mu\) ≠ 80 \(\overline{x}\) = 88, \(\mu\) = 80, n = 36, \(\sigma\) = 10. \(\alpha\) = 0.05 / 2 = 0.025 The critical value using the normal distribution table is 1.96 z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) z = \(\frac{88-80}{\frac{10}{\sqrt{36}}}\) = 4.8 As 4.8 > 1.96, the null hypothesis is rejected. Answer: There is a difference in the scores after the new curriculum was introduced.
• Example 3: The average score of a class is 90. However, a teacher believes that the average score might be lower. The scores of 6 students were randomly measured. The mean was 82 with a standard deviation of 18. With a 0.05 significance level use hypothesis testing to check if this claim is true. Solution: The t test will be used. \(H_{0}\): \(\mu\) = 90, \(H_{1}\): \(\mu\) < 90 \(\overline{x}\) = 110, \(\mu\) = 90, n = 6, s = 18 The critical value from the t table is -2.015 t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\) t = \(\frac{82-90}{\frac{18}{\sqrt{6}}}\) t = -1.088 As -1.088 > -2.015, we fail to reject the null hypothesis. Answer: There is not enough evidence to support the claim.

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FAQs on Hypothesis Testing

What is hypothesis testing.

Hypothesis testing in statistics is a tool that is used to make inferences about the population data. It is also used to check if the results of an experiment are valid.

What is the z Test in Hypothesis Testing?

The z test in hypothesis testing is used to find the z test statistic for normally distributed data . The z test is used when the standard deviation of the population is known and the sample size is greater than or equal to 30.

What is the t Test in Hypothesis Testing?

The t test in hypothesis testing is used when the data follows a student t distribution . It is used when the sample size is less than 30 and standard deviation of the population is not known.

What is the formula for z test in Hypothesis Testing?

The formula for a one sample z test in hypothesis testing is z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) and for two samples is z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).

What is the p Value in Hypothesis Testing?

The p value helps to determine if the test results are statistically significant or not. In hypothesis testing, the null hypothesis can either be rejected or not rejected based on the comparison between the p value and the alpha level.

What is One Tail Hypothesis Testing?

When the rejection region is only on one side of the distribution curve then it is known as one tail hypothesis testing. The right tail test and the left tail test are two types of directional hypothesis testing.

What is the Alpha Level in Two Tail Hypothesis Testing?

To get the alpha level in a two tail hypothesis testing divide \(\alpha\) by 2. This is done as there are two rejection regions in the curve.

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Hypothesis Testing: 4 Steps and Example

Hypothesis testing, sometimes called significance testing, is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used and the reason for the analysis.

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data. Such data may come from a larger population or a data-generating process. The word "population" will be used for both of these cases in the following descriptions.

Key Takeaways

• Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data.
• The test provides evidence concerning the plausibility of the hypothesis, given the data.
• Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed.
• The four steps of hypothesis testing include stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

How Hypothesis Testing Works

In hypothesis testing, an  analyst  tests a statistical sample, intending to provide evidence on the plausibility of the null hypothesis. Statistical analysts measure and examine a random sample of the population being analyzed. All analysts use a random population sample to test two different hypotheses: the null hypothesis and the alternative hypothesis.

The null hypothesis is usually a hypothesis of equality between population parameters; e.g., a null hypothesis may state that the population mean return is equal to zero. The alternative hypothesis is effectively the opposite of a null hypothesis. Thus, they are mutually exclusive , and only one can be true. However, one of the two hypotheses will always be true.

The null hypothesis is a statement about a population parameter, such as the population mean, that is assumed to be true.

• State the hypotheses.
• Formulate an analysis plan, which outlines how the data will be evaluated.
• Carry out the plan and analyze the sample data.
• Analyze the results and either reject the null hypothesis, or state that the null hypothesis is plausible, given the data.

Example of Hypothesis Testing

If an individual wants to test that a penny has exactly a 50% chance of landing on heads, the null hypothesis would be that 50% is correct, and the alternative hypothesis would be that 50% is not correct. Mathematically, the null hypothesis is represented as Ho: P = 0.5. The alternative hypothesis is shown as "Ha" and is identical to the null hypothesis, except with the equal sign struck-through, meaning that it does not equal 50%.

A random sample of 100 coin flips is taken, and the null hypothesis is tested. If it is found that the 100 coin flips were distributed as 40 heads and 60 tails, the analyst would assume that a penny does not have a 50% chance of landing on heads and would reject the null hypothesis and accept the alternative hypothesis.

If there were 48 heads and 52 tails, then it is plausible that the coin could be fair and still produce such a result. In cases such as this where the null hypothesis is "accepted," the analyst states that the difference between the expected results (50 heads and 50 tails) and the observed results (48 heads and 52 tails) is "explainable by chance alone."

When Did Hypothesis Testing Begin?

Some statisticians attribute the first hypothesis tests to satirical writer John Arbuthnot in 1710, who studied male and female births in England after observing that in nearly every year, male births exceeded female births by a slight proportion. Arbuthnot calculated that the probability of this happening by chance was small, and therefore it was due to “divine providence.”

What are the Benefits of Hypothesis Testing?

Hypothesis testing helps assess the accuracy of new ideas or theories by testing them against data. This allows researchers to determine whether the evidence supports their hypothesis, helping to avoid false claims and conclusions. Hypothesis testing also provides a framework for decision-making based on data rather than personal opinions or biases. By relying on statistical analysis, hypothesis testing helps to reduce the effects of chance and confounding variables, providing a robust framework for making informed conclusions.

What are the Limitations of Hypothesis Testing?

Hypothesis testing relies exclusively on data and doesn’t provide a comprehensive understanding of the subject being studied. Additionally, the accuracy of the results depends on the quality of the available data and the statistical methods used. Inaccurate data or inappropriate hypothesis formulation may lead to incorrect conclusions or failed tests. Hypothesis testing can also lead to errors, such as analysts either accepting or rejecting a null hypothesis when they shouldn’t have. These errors may result in false conclusions or missed opportunities to identify significant patterns or relationships in the data.

Hypothesis testing refers to a statistical process that helps researchers determine the reliability of a study. By using a well-formulated hypothesis and set of statistical tests, individuals or businesses can make inferences about the population that they are studying and draw conclusions based on the data presented. All hypothesis testing methods have the same four-step process, which includes stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

Sage. " Introduction to Hypothesis Testing ," Page 4.

Elder Research. " Who Invented the Null Hypothesis? "

Formplus. " Hypothesis Testing: Definition, Uses, Limitations and Examples ."

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Statistics Made Easy

5 Tips for Interpreting P-Values Correctly in Hypothesis Testing

Hypothesis testing is a critical part of statistical analysis and is often the endpoint where conclusions are drawn about larger populations based on a sample or experimental dataset. Central to this process is the p-value. Broadly, the p-value quantifies the strength of evidence against the null hypothesis. Given the importance of the p-value, it is essential to ensure its interpretation is correct. Here are five essential tips for ensuring the p-value from a hypothesis test is understood correctly. 

1. Know What the P-value Represents

First, it is essential to understand what a p-value is. In hypothesis testing, the p-value is defined as the probability of observing your data, or data more extreme, if the null hypothesis is true. As a reminder, the null hypothesis states no difference between your data and the expected population. 

For example, in a hypothesis test to see if changing a company’s logo drives more traffic to the website, a null hypothesis would state that the new traffic numbers are equal to the old traffic numbers. In this context, the p-value would be the probability that the data you observed, or data more extreme, would occur if this null hypothesis were true. 

Therefore, a smaller p-value indicates that what you observed is unlikely to have occurred if the null were true, offering evidence to reject the null hypothesis. Typically, a cut-off value of 0.05 is used where any p-value below this is considered significant evidence against the null. 

2. Understand the Directionality of Your Hypothesis

Based on the research question under exploration, there are two types of hypotheses: one-sided and two-sided. A one-sided test specifies a particular direction of effect, such as traffic to a website increasing after a design change. On the other hand, a two-sided test allows the change to be in either direction and is effective when the researcher wants to see any effect of the change. 

Either way, determining the statistical significance of a p-value is the same: if the p-value is below a threshold value, it is statistically significant. However, when calculating the p-value, it is important to ensure the correct sided calculations have been completed. 

Additionally, the interpretation of the meaning of a p-value will differ based on the directionality of the hypothesis. If a one-sided test is significant, the researchers can use the p-value to support a statistically significant increase or decrease based on the direction of the test. If a two-sided test is significant, the p-value can only be used to say that the two groups are different, but not that one is necessarily greater. 

3. Avoid Threshold Thinking

A common pitfall in interpreting p-values is falling into the threshold thinking trap. The most commonly used cut-off value for whether a calculated p-value is statistically significant is 0.05. Typically, a p-value of less than 0.05 is considered statistically significant evidence against the null hypothesis. 

However, this is just an arbitrary value. Rigid adherence to this or any other predefined cut-off value can obscure business-relevant effect sizes. For example, a hypothesis test looking at changes in traffic after a website design may find that an increase of 10,000 views is not statistically significant with a p-value of 0.055 since that value is above 0.05. However, the actual increase of 10,000 may be important to the growth of the business. 

Therefore, a p-value can be practically significant while not being statistically significant. Both types of significance and the broader context of the hypothesis test should be considered when making a final interpretation. 

4. Consider the Power of Your Study

Similarly, some study conditions can result in a non-significant p-value even if practical significance exists. Statistical power is the ability of a study to detect an effect when it truly exists. In other words, it is the probability that the null hypothesis will be rejected when it is false. 

Power is impacted by a lot of factors. These include sample size, the effect size you are looking for, and variability within the data. In the example of website traffic after a design change, if the number of visits overall is too small, there may not be enough views to have enough power to detect a difference. 

Simple ways to increase the power of a hypothesis test and increase the chances of detecting an effect are increasing the sample size, looking for a smaller effect size, changing the experiment design to control for variables that can increase variability, or adjusting the type of statistical test being run.

5. Be Aware of Multiple Comparisons

Whenever multiple p-values are calculated in a single study due to multiple comparisons, there is an increased risk of false positives. This is because each individual comparison introduces random fluctuations, and each additional comparison compounds these fluctuations. 

For example, in a hypothesis test looking at traffic before and after a website redesign, the team may be interested in making more than one comparison. This can include total visits, page views, and average time spent on the website. Since multiple comparisons are being made, there must be a correction made when interpreting the p-value. 

The Bonferroni correction is one of the most commonly used methods to account for this increased probability of false positives. In this method, the significance cut-off value, typically 0.05, is divided by the number of comparisons made. The result is used as the new significance cut-off value.  Applying this correction mitigates the risk of false positives and improves the reliability of findings from a hypothesis test. 

In conclusion, interpreting p-values requires a nuanced understanding of many statistical concepts and careful consideration of the hypothesis test’s context. By following these five tips, the interpretation of the p-value from a hypothesis test can be more accurate and reliable, leading to better data-driven decision-making.

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Scientists are testing mRNA vaccines to protect cows and people against bird flu

T he bird flu outbreak in U.S. dairy cows is prompting development of new, next-generation mRNA vaccines — akin to COVID-19 shots — that are being tested in both animals and people.

Next month, the U.S. Agriculture Department is to begin testing a vaccine developed by University of Pennsylvania researchers by giving it to calves. The idea: If vaccinating cows protects dairy workers, that could mean fewer chances for the virus to jump into people and mutate in ways that could spur human-to-human spread.

Meanwhile. the U.S. Department of Health and Human Services has been talking to manufacturers about possible mRNA flu vaccines for people that, if needed, could supplement millions of bird flu vaccine doses already in government hands.

“If there's a pandemic, there's going to be a huge demand for vaccine,” said Richard Webby, a flu researcher at St. Jude Children’s Research Hospital in Memphis. “The more different (vaccine manufacturing) platforms that can respond to that, the better."

The bird flu virus has been spreading among more animal species in scores of countries since 2020. It was detected in U.S. dairy herds in March, although investigators think it may have been in cows since December. This week, the USDA announced it had been found in alpacas for the first time.

At least three people — all workers at farms with infected cows — have been diagnosed with bird flu, although the illnesses were considered mild.

But earlier versions of the same H5N1 flu virus have been highly lethal to humans in other parts of the world. Officials are taking steps to be prepared if the virus mutates in a way to make it more deadly or enables it to spread more easily from person to person.

Traditionally, most flu vaccines are made via an egg-based manufacturing process that's been used for more than 70 years. It involves injecting a candidate virus into fertilized chicken eggs, which are incubated for several days to allow the viruses to grow. Fluid is harvested from the eggs and is used as the basis for vaccines, with killed or weakened virus priming the body's immune system.

Rather than eggs — also vulnerable to bird flu-caused supply constraints — some flu vaccine is made in giant vats of cells.

Officials say they already have two candidate vaccines for people that appear to be well-matched to the bird flu virus in U.S. dairy herds. The Centers for Disease Control and Prevention used the circulating bird flu virus as the seed strain for them.

The government has hundreds of thousands of vaccine doses in pre-filled syringes and vials that likely could go out in a matter of weeks, if needed, federal health officials say.

They also say they have bulk antigen that could generate nearly 10 million more doses that could be filled, finished and distributed in a matter of a few months. CSL Seqirus, which manufactures cell-based flu vaccine, this week announced that the government hired it to fill and finish about 4.8 million of those doses. The work could be done by late summer, U.S. health officials said this week.

But the production lines for flu vaccines are already working on this fall's seasonal shots — work that would have to be interrupted to produce millions more doses of bird flu vaccine. So the government has been pursuing another, quicker approach: the mRNA technology used to produce the primary vaccines deployed against COVID-19.

These messenger RNA vaccines are made using a small section of genetic material from the virus. The genetic blueprint is designed to teach the body how to make a protein used to build immunity.

The pharmaceutical company Moderna already has a bird flu mRNA vaccine in very early-stage human testing. In a statement, Moderna confirmed that “we are in discussions with the U.S. government on advancing our pandemic flu candidate."

Similar work has been going on at Pfizer. Company researchers in December gave human volunteers an mRNA vaccine against a bird flu strain that's similar to — but not exactly the same as — the one in cows. Since then, researchers have performed a lab experiment exposing blood samples from those volunteers to the strain seen in dairy farms, and saw a “notable increases in antibody responses," Pfizer said in a statement.

As for the vaccine for cows, Penn immunologist Scott Hensley worked with mRNA pioneer and Nobel laureate Drew Weissman to produce the experimental doses. Hensley said that vaccine is similar to the Moderna one for people.

In first-step testing, mice and ferrets produced high levels of bird flu virus-fighting antibodies after vaccination.

In another experiment, researchers vaccinated one group of ferrets and deliberately infected them, and then compared what happened to ferrets that hadn't been vaccinated. All the vaccinated animals survived and the unvaccinated did not, Hensley said.

“The vaccine was really successful,” said Webby, whose lab did that work last year in collaboration with Hensley.

The cow study will be akin to the first-step testing initially done in smaller animals. The plan is for initially about 10 calves to be vaccinated, half with one dose and half with another. Then their blood will be drawn and examined to look for how much bird flu-fighting antibodies were produced.

The USDA study first will have to determine the right dose for such a large animal, Hensley said, before testing if it protects them like it did smaller animals.

What “scares me the most is the amount of interaction between cattle and humans,” Hensley said.

“We’re not talking about an animal that lives on a mountain top," he said. "If this was a bobcat outbreak I’d feel bad for the bobcats, but that’s not a big human risk.”

If a vaccine reduces the amount of virus in the cow, “then ultimately we reduce the chance that a mutant virus that spreads in humans is going to emerge,” he said.

The Associated Press Health and Science Department receives support from the Howard Hughes Medical Institute’s Science and Educational Media Group. The AP is solely responsible for all content.

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10.29: Hypothesis Test for a Difference in Two Population Means (1 of 2)

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Learning Objectives

• Under appropriate conditions, conduct a hypothesis test about a difference between two population means. State a conclusion in context.

Using the Hypothesis Test for a Difference in Two Population Means

The general steps of this hypothesis test are the same as always. As expected, the details of the conditions for use of the test and the test statistic are unique to this test (but similar in many ways to what we have seen before.)

Step 1: Determine the hypotheses.

The hypotheses for a difference in two population means are similar to those for a difference in two population proportions. The null hypothesis, H 0 , is again a statement of “no effect” or “no difference.”

• H 0 : μ 1 – μ 2 = 0, which is the same as H 0 : μ 1 = μ 2

The alternative hypothesis, H a , can be any one of the following.

• H a : μ 1 – μ 2 < 0, which is the same as H a : μ 1 < μ 2
• H a : μ 1 – μ 2 > 0, which is the same as H a : μ 1 > μ 2
• H a : μ 1 – μ 2 ≠ 0, which is the same as H a : μ 1 ≠ μ 2

Step 2: Collect the data.

As usual, how we collect the data determines whether we can use it in the inference procedure. We have our usual two requirements for data collection.

• Samples must be random to remove or minimize bias.
• Samples must be representative of the populations in question.

We use this hypothesis test when the data meets the following conditions.

• The two random samples are independent .
• The variable is normally distributed in both populations . If this variable is not known, samples of more than 30 will have a difference in sample means that can be modeled adequately by the t-distribution. As we discussed in “Hypothesis Test for a Population Mean,” t-procedures are robust even when the variable is not normally distributed in the population. If checking normality in the populations is impossible, then we look at the distribution in the samples. If a histogram or dotplot of the data does not show extreme skew or outliers, we take it as a sign that the variable is not heavily skewed in the populations, and we use the inference procedure. (Note: This is the same condition we used for the one-sample t-test in “Hypothesis Test for a Population Mean.”)

Step 3: Assess the evidence.

If the conditions are met, then we calculate the t-test statistic. The t-test statistic has a familiar form.

Since the null hypothesis assumes there is no difference in the population means, the expression (μ 1 – μ 2 ) is always zero.

As we learned in “Estimating a Population Mean,” the t-distribution depends on the degrees of freedom (df) . In the one-sample and matched-pair cases df = n – 1. For the two-sample t-test, determining the correct df is based on a complicated formula that we do not cover in this course. We will either give the df or use technology to find the df . With the t-test statistic and the degrees of freedom, we can use the appropriate t-model to find the P-value, just as we did in “Hypothesis Test for a Population Mean.” We can even use the same simulation.

Step 4: State a conclusion.

To state a conclusion, we follow what we have done with other hypothesis tests. We compare our P-value to a stated level of significance.

• If the P-value ≤ α, we reject the null hypothesis in favor of the alternative hypothesis.
• If the P-value > α, we fail to reject the null hypothesis. We do not have enough evidence to support the alternative hypothesis.

As always, we state our conclusion in context, usually by referring to the alternative hypothesis.

“Context and Calories”

Does the company you keep impact what you eat? This example comes from an article titled “Impact of Group Settings and Gender on Meals Purchased by College Students” (Allen-O’Donnell, M., T. C. Nowak, K. A. Snyder, and M. D. Cottingham, Journal of Applied Social Psychology 49(9), 2011, onlinelibrary.wiley.com/doi/10.1111/j.1559-1816.2011.00804.x/full) . In this study, researchers examined this issue in the context of gender-related theories in their field. For our purposes, we look at this research more narrowly.

Step 1: Stating the hypotheses.

In the article, the authors make the following hypothesis. “The attempt to appear feminine will be empirically demonstrated by the purchase of fewer calories by women in mixed-gender groups than by women in same-gender groups.” We translate this into a simpler and narrower research question: Do women purchase fewer calories when they eat with men compared to when they eat with women?

Here the two populations are “women eating with women” (population 1) and “women eating with men” (population 2). The variable is the calories in the meal. We test the following hypotheses at the 5% level of significance.

The null hypothesis is always H 0 : μ 1 – μ 2 = 0, which is the same as H 0 : μ 1 = μ 2 .

The alternative hypothesis H a : μ 1 – μ 2 > 0, which is the same as H a : μ 1 > μ 2 .

Here μ 1 represents the mean number of calories ordered by women when they were eating with other women, and μ 2 represents the mean number of calories ordered by women when they were eating with men.

Note: It does not matter which population we label as 1 or 2, but once we decide, we have to stay consistent throughout the hypothesis test. Since we expect the number of calories to be greater for the women eating with other women, the difference is positive if “women eating with women” is population 1. If you prefer to work with positive numbers, choose the group with the larger expected mean as population 1. This is a good general tip.

Step 2: Collect Data.

As usual, there are two major things to keep in mind when considering the collection of data.

• Samples need to be representative of the population in question.
• Samples need to be random in order to remove or minimize bias.

Representative Samples?

The researchers state their hypothesis in terms of “women.” We did the same. But the researchers gathered data by watching people eat at the HUB Rock Café II on the campus of Indiana University of Pennsylvania during the Spring semester of 2006. Almost all of the women in the data set were white undergraduates between the ages of 18 and 24, so there are some definite limitations on the scope of this study. These limitations will affect our conclusion (and the specific definition of the population means in our hypotheses.)

Random Samples?

The observations were collected on February 13, 2006, through February 22, 2006, between 11 a.m. and 7 p.m. We can see that the researchers included both lunch and dinner. They also made observations on all days of the week to ensure that weekly customer patterns did not confound their findings. The authors state that “since the time period for observations and the place where [they] observed students were limited, the sample was a convenience sample.” Despite these limitations, the researchers conducted inference procedures with the data, and the results were published in a reputable journal. We will also conduct inference with this data, but we also include a discussion of the limitations of the study with our conclusion. The authors did this, also.

Do the data met the conditions for use of a t-test?

The researchers reported the following sample statistics.

• In a sample of 45 women dining with other women, the average number of calories ordered was 850, and the standard deviation was 252.
• In a sample of 27 women dining with men, the average number of calories ordered was 719, and the standard deviation was 322.

One of the samples has fewer than 30 women. We need to make sure the distribution of calories in this sample is not heavily skewed and has no outliers, but we do not have access to a spreadsheet of the actual data. Since the researchers conducted a t-test with this data, we will assume that the conditions are met. This includes the assumption that the samples are independent.

As noted previously, the researchers reported the following sample statistics.

To compute the t-test statistic, make sure sample 1 corresponds to population 1. Here our population 1 is “women eating with other women.” So x 1 = 850, s 1 = 252, n 1 =45, and so on.

Using technology, we determined that the degrees of freedom are about 45 for this data. To find the P-value, we use our familiar simulation of the t-distribution. Since the alternative hypothesis is a “greater than” statement, we look for the area to the right of T = 1.81. The P-value is 0.0385.

Generic Conclusion

The hypotheses for this test are H 0 : μ 1 – μ 2 = 0 and H a : μ 1 – μ 2 > 0. Since the P-value is less than the significance level (0.0385 < 0.05), we reject H 0 and accept H a .

Conclusion in context

At Indiana University of Pennsylvania, the mean number of calories ordered by undergraduate women eating with other women is greater than the mean number of calories ordered by undergraduate women eating with men (P-value = 0.0385).

Comment about Conclusions

In the conclusion above, we did not generalize the findings to all women. Since the samples included only undergraduate women at one university, we included this information in our conclusion. But our conclusion is a cautious statement of the findings. The authors see the results more broadly in the context of theories in the field of social psychology. In the context of these theories, they write, “Our findings support the assertion that meal size is a tool for influencing the impressions of others. For traditional-age, predominantly White college women, diminished meal size appears to be an attempt to assert femininity in groups that include men.” This viewpoint is echoed in the following summary of the study for the general public on National Public Radio (npr.org).

• Both men and women appear to choose larger portions when they eat with women, and both men and women choose smaller portions when they eat in the company of men, according to new research published in the Journal of Applied Social Psychology . The study, conducted among a sample of 127 college students, suggests that both men and women are influenced by unconscious scripts about how to behave in each other’s company. And these scripts change the way men and women eat when they eat together and when they eat apart.

Should we be concerned that the findings of this study are generalized in this way? Perhaps. But the authors of the article address this concern by including the following disclaimer with their findings: “While the results of our research are suggestive, they should be replicated with larger, representative samples. Studies should be done not only with primarily White, middle-class college students, but also with students who differ in terms of race/ethnicity, social class, age, sexual orientation, and so forth.” This is an example of good statistical practice. It is often very difficult to select truly random samples from the populations of interest. Researchers therefore discuss the limitations of their sampling design when they discuss their conclusions.

In the following activities, you will have the opportunity to practice parts of the hypothesis test for a difference in two population means. On the next page, the activities focus on the entire process and also incorporate technology.

National Health and Nutrition Survey

https://assessments.lumenlearning.co...sessments/3705

https://assessments.lumenlearning.co...sessments/3782

https://assessments.lumenlearning.co...sessments/3706

Contributors and Attributions

• Concepts in Statistics. Provided by : Open Learning Initiative. Located at : http://oli.cmu.edu . License : CC BY: Attribution

News | City of Hope’s new blood test for lung cancer…

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News | City of Hope’s new blood test for lung cancer will mean more early detections

A mobile clinic in antelope valley and a test site in pomona launch a testing and detection study.

The City of Hope and DELFI Diagnostics Inc. are trying out a new kind of screening for lung cancer that uses a simple blood test. They believe that this much more convenient and less costly testing will draw in more people for screening, and save lives through early detection and treatment.

“It is very frustrating to see such low adoption of lung cancer screening,” said Dr. Dan Raz, a thoracic surgeon at City of Hope in Duarte and director of the lung cancer screening program. “This test has a lot of potential, especially for people who have a lot of barriers to getting screened.”

Lung cancer kills more people than any other kind of cancer. About 150,000 deaths each year in the U.S. are from lung cancer alone. But about 98% of those eligible for the scan in California are not getting it. In the U.S., 94% are eligible but only about 6% are being scanned, according to City of Hope and its partner, DELFI.

Blood test will save lives

“It’s important because it will save lives through lung cancer early detection. Finding it early will reduce the chances people will die,” explained Dr. Peter Bach, a former pulmonary and intensive care physician at Memorial Sloan Cancer Center in New York and now the chief medical officer at DELFI.

“The challenge we have had, even though (a CT scan) is covered by insurance, it is not particularly convenient and has not been used at rates of other screening tests” for other types of cancer, Bach said.

The CT scans are often hit by delays, plus the lack of same-day or weekend testing. The scans take several hours or often half a day, and many people can’t or won’t take time off from work. Health insurance delays and transportation to a hospital or clinic also create barriers, Raz said.

Getting the right preventative care at the right time can mean the difference between life and death. Lung cancer is often found by accident, while a patient is getting treatment for unrelated conditions, Raz said.

“We know that if we successfully get to patients, our tests will help identify who should get a CT scan. It can prevent 10,000 deaths in the United States if fully implemented,” Bach said.

Testing in Antelope Valley and Pomona

So when City of Hope teamed up with DELFI, they began focusing on high-risk populations, such as those who smoked cigarettes for years, as well as low-income socio-economic populations with barriers to healthcare.

Starting in late April, a mobile testing unit was sent to the Antelope Valley. The team is also signing up people for the blood test at the ParkTree Community Health Center in Pomona. The test is offered free of charge to eligible trial participants.

Other areas are also beginning trials. In Baltimore, Johns Hopkins Hospital has begun a similar pilot program, said Dr. Panagis Galiatsatos, a pulmonary and critical care doctor who is a co-investigator of the DELFI blood test at Johns Hopkins and a spokesperson for the American Lung Association.

Galiatsatos said the blood test adds another tool for detecting lung cancer. “The intention is to make lung cancer diagnosis better,” he said on Wednesday, May 29. “So having a biomarker (from the blood test) can get at cancer in real time. It gives clinicians a bit more confidence when we talk to patients.”

Here’s how it works

First, doctors say it’s important to note that the blood test tells patients they may have lung cancer. It does not tell people they have cancer. Instead, for those found to have biomarkers, doctors direct them to get a CT scan, the gold standard of lung cancer screening.

The blood test can be done in conjunction with other doctor visits for blood screenings, for cholesterol or blood sugar levels, for example. Only one vial of blood is needed. And City of Hope can send a team to your home if you don’t have transportation, Raz said.

Clinicians look at genetic material specific to lung cancer, Raz said. “It looks for lung cancer cells and the patterns of fragments of DNA” floating in the blood, he explained.

“It’s like Where’s Waldo,” said Dr. Bach with DELFI. “We are looking at the way the DNA has been chewed up into fragments.”

Battling public resistance

But as with many new inventions, getting the general public to accept them can be a challenge.

People in Black communities may not trust doctors or government tests, said Rich Wallace, president and CEO of the Southern California Black Chamber of Commerce.

“These people don’t want to be tested. Black people don’t trust doctors. They also don’t want to be a guinea pig,” said Wallace on May 28.

Testing by the government in a 1932 study originally called the “Tuskegee Study of Untreated Syphilis in the Negro Male” of about 600 Black men, including 201 who did not have the disease, was done without the participants’ informed consent, according to the Centers for Disease Control and Prevention. In 1972 the study was called “unethically justified.”

“We have a distrust especially when we hear the word ‘test,’ ” Wallace said.

Darryl Jackson, pastor of Love Chapel Life Changing Ministries in Ontario, said he and his congregants are not worried about being tested. They were part of a five-year Inland Empire Smoke Out program in which they learned about the dangers of smoking and the fact that it causes lung cancer. His congregation is 95% smoke-free.

“Yeah, I think that may be something my people would be interested in,” Jackson said. “Something where people can get a blood test and then, especially if they can catch it early.” He said he would tell his congregation to go to the clinic in nearby Pomona.

City of Hope and DELFI are aware of the reasons that prevent people from signing up, and that’s part of the program — providing ways to break down barriers.

Some who have smoked for many years see that as a stigma and don’t want to admit it, according to surveys done at the clinic sites, said Raz. “They say ‘I am afraid of being judged for having smoked,’ ” he said. Another barrier is that people just don’t want to know.

“In the Black community, for some reason, we have a concept that goes like this: ‘If I don’t go, I won’t know.’ There is a fear of doctors,” Jackson said. In the program, he heard from smokers and those who’ve quit who say they will just wait and see what happens. “It can be procrastination in taking care of our bodies.”

Raz said that often when symptoms arise, it is too late to save the patient’s life.

He said they will provide transportation to the blood test site and to the CT scan appointment. They will work with Medi-Cal patients for clearance, or with employers to provide a day off. If the participant doesn’t have insurance, they can help pay the cost of the CT scan.

Overcoming social resistance to tests, doctors and scans takes a constant effort, especially in low-income, minority communities, said Galiatsatos. But it can be done by talking first to influencers such as rabbis, priests, pastors and imams, who in turn inform their congregants.

“You do it with good advocacy,” he said. “We must help individuals overcome social factors in order to keep them achieving the health they are promised.”

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What the Different Levels of Cortisol Mean on Your Test

Master the understanding of different cortisol levels during testing, empowering you to better manage your health and overall well-being.

Ellen Landes, MS, RDN, CPT, owner of The Runner's Dietitian, optimizes runners' performance and well-being through realistic nutrition. A registered dietitian and certified personal trainer, Ellen combines expertise in exercise science and dietary principles.

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With over 7 years of editing in the scientific research industry, Caitlin specializes in making complex topics accessible. Drawing on her background in Visual Communication Design, she uses a creative approach to provide clear and engaging content.

Published June 3, 2024.

Feeling constantly wiped out or inexplicably jittery? These energy imbalances could be a sign of cortisol dysfunction.

Maintaining optimal cortisol can support various bodily functions such as metabolism and immune responses, suppress inflammation , and regulate blood sugar. That's why we'll explore the causes and implications of high and low levels to keep them in a healthy range.

Causes of high cortisol levels

Cortisol, often called the "stress hormone," naturally follows a rhythm throughout the day. In general, the normal range for cortisol levels in the blood is between 10-20 micrograms per deciliter (mcg/dL).

The highest levels are typically seen in the morning, peaking between 6 and 8 a.m. This is known as the cortisol awakening response (CAR) and is a part of the body's natural rhythm. [2] They then decrease, reaching their lowest point in the evening, usually between 11 p.m. and midnight.

But, various factors can disrupt this rhythm, such as:

As we age, our body's stress response system, the hypothalamic-pituitary-adrenal (HPA) axis, becomes less fine-tuned. This can lead to two critical changes in cortisol, the body's primary stress hormone:

• Slightly blunted cortisol awakening response:  The morning surge of cortisol helps us wake up and feel energized, maybe a little lower in older adults. [3]
• Higher average cortisol levels throughout the day:  Even though the peak is lower, the body might not switch off cortisol production as effectively throughout the day, leading to slightly elevated levels.

Certain medications can significantly impact cortisol levels by directly affecting the adrenal glands which produce it. For example, prednisone mimics the hormone's effects, which can suppress natural production. [4]

Some antidepressants—particularly those that affect serotonin levels—can also interact with the body's stress response system and alter cortisol production. [5]

Acute and chronic stress

Understanding the nuanced impact of stress on cortisol levels involves distinguishing between acute—short-term—and chronic or long-term stress responses.

Acute stress, like a public speaking event or a tight deadline, leads to a temporary—within minutes to an hour—increase in the hormone's levels. This natural and adaptive response, known as a cortisol surge, helps the body react quickly and effectively.

On the other hand, when stressors become chronic or unrelenting, cortisol levels can stay elevated instead of dipping back down like they usually do. This sustained surge can disrupt the body's systems, impacting your overall health. [6]

Lifestyle factors

Inconsistent sleep schedules, insufficient sleep duration—less than 7-8 hours—and poor sleep quality can all contribute to elevated cortisol. [7] Regularly spiking your blood sugar with sweets or refined carbohydrates can also contribute, as the body releases this hormone to fight the rising insulin.

» Discover the connection between cortisol, creatine kinase, and stress 

Adrenal disorders

Several adrenal gland disorders can also lead to high cortisol levels. These include Cushing syndrome, which can be caused by tumors or long-term use of corticosteroid medications [8]

Other examples are primary aldosteronism, affecting blood pressure and potassium levels, and congenital adrenal hyperplasia, a genetic condition. One common cause of high cortisol from adrenal gland tumors is an adrenal adenoma. [9, 10]

Implications of high cortisol levels

Over time, high levels can lead to various health issues, such as:

• An increased risk of metabolic syndrome characterized by abdominal fat accumulation.
•  High blood pressure and impaired blood glucose regulation lead to conditions like diabetes.
• Suppression of the immune system reduces the production of white blood cells and their ability to fight infections, increasing susceptibility to illness.
• Mood disorders such as anxiety, depression , and other mental health conditions.

Factors influencing low cortisol levels

Low cortisol levels can occur due to adrenal insufficiency, which can be categorized as primary, secondary, or tertiary.

Primary: This type results from dysfunction of the adrenal glands themselves, where they fail to produce enough cortisol. Addison's disease is a typical example. Autoimmune diseases, infections, or direct gland damage can cause this type.

Secondary: This occurs when the pituitary gland, located at the base of the brain, is impaired. This disrupts the normal signaling to the adrenal glands, leading to insufficient cortisol production.

Tertiary: The rarest form that involves damage to the hypothalamus, a region above the pituitary gland. This damage disrupts its ability to signal the pituitary gland, which in turn, cannot stimulate cortisol production by the adrenal glands.

Note: InsideTracker’s Ultimate plan will analyze blood biomarkers to help you understand your current cortisol health. It will also determine the ideal range based on your lifestyle and demographic, offering specific nutrition and lifestyle recommendations.

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InsideTracker is a personalized health and wellness platform that aims to help people optimize their biomarkers to live healthier and longer lives. It analyzes genetics, behaviors, and goals to give you personalized nutrition, fitness, sleep, stress, and supplementation recommendations.

Why choose InsideTracker

InsideTracker offers DNA testing for dozens of genetic fitness, nutrition, and longevity genetic markers. Since genetics influence many aspects of your health, the app can provide helpful context and an action plan. It also integrates with wearable devices to collect real-time health data, tracking factors like sleep, activity, and heart rate.

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Science-backed recommendations

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Encourages retesting every 3 to 6 months

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Not a replacement for medical care

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Implications of low cortisol levels

Chronically low cortisol levels can lead to health complications. If your body doesn't produce enough, you may experience symptoms like fatigue , muscle weakness, and weight fluctuations. Additionally, you may have issues with blood sugar regulation and high blood pressure.

» Explore the benefits of blood testing for optimal health

Testing cortisol abnormalities

Healthcare providers assess cortisol levels, considering individual differences and natural daily fluctuations. Blood, urine, and saliva tests help them diagnose underlying medical issues.

For instance, high levels detected in these tests may indicate Cushing's syndrome, while low levels could suggest adrenal insufficiency. Imaging techniques like X-rays and CT scans can further pinpoint causes like tumors or adrenal gland abnormalities that affect cortisol production. [11]

Possible treatment options

If a cortisol deficiency is identified, healthcare providers will likely recommend replacement therapy. This treatment involves medications like hydrocortisone or prednisone, which mimic the body's natural production.

Chronic stress is a significant contributor to high cortisol levels, but genetics and underlying health conditions can also play a role. To manage it, doctors may recommend lifestyle modifications like stress management techniques, regular exercise, and a balanced diet.

Note: Each situation warrants a tailored approach. It's crucial to consult a healthcare professional for individualized treatment plans.

» Learn how to interpret your blood test results 

Take charge: navigating cortisol levels for optimal health

The field of cortisol management is rapidly evolving, with ongoing research exploring genetics, lifestyle factors, and the brain-body connection to stress and hormones [10].

Exciting new treatment possibilities are being explored, including medications, gene therapy, and even AI. Its potential to predict and personalize treatment plans offers a glimpse into a future where cortisol management is more precise and effective.

For example, InsideTracker uses algorithms to interpret health data and identify personalized optimal cortisol levels and factors like age, sex, and ethnicity to offer tailored recommendations for improving overall health.

Reference list:

[1] L. Thau, J. Gandhi, and S. Sharma, “Physiology, cortisol,” StatPearls - NCBI Bookshelf , Aug. 28, 2023. Available: https://www.ncbi.nlm.nih.gov/books/NBK538239/ 

[2] N. Bowles et al. , “The circadian system modulates the cortisol awakening response in humans,” Frontiers in Neuroscience , vol. 16, Nov. 2022, doi: 10.3389/fnins.2022.995452. Available: https://pubmed.ncbi.nlm.nih.gov/36408390/ 

[3] E. Van Cauter, R. Leproult, and D. J. Kupfer, “Effects of gender and age on the levels and circadian rhythmicity of plasma cortisol.,” The Journal of Clinical Endocrinology & Metabolism , vol. 81, no. 7, pp. 2468–2473, Jul. 1996, doi: 10.1210/jcem.81.7.8675562. Available: https://pubmed.ncbi.nlm.nih.gov/8675562/ 

[4] “Corticosteroids,” PubMed , Jan. 01, 2024. Available: https://pubmed.ncbi.nlm.nih.gov/32119499/ 

[5] A. Subramaniam, A. M. LoPilato, and E. F. Walker, “Psychotropic medication effects on cortisol: Implications for research and mechanisms of drug action,” Schizophrenia Research (Print) , vol. 213, pp. 6–14, Nov. 2019, doi: 10.1016/j.schres.2019.06.023. Available: https://pubmed.ncbi.nlm.nih.gov/31307858/ 

[6] K. Hannibal and M. D. Bishop, “Chronic Stress, cortisol dysfunction, and pain: A Psychoneuroendocrine Rationale for Stress management in Pain Rehabilitation,” Physical Therapy , vol. 94, no. 12, pp. 1816–1825, Dec. 2014, doi: 10.2522/ptj.20130597. Available: https://pubmed.ncbi.nlm.nih.gov/25035267/ 

[7] A. H. Garde et al. , “Effects of lifestyle factors on concentrations of salivary cortisol in healthy individuals,” Scandinavian Journal of Clinical & Laboratory Investigation , vol. 69, no. 2, pp. 242–250, Jan. 2009, doi: 10.1080/00365510802483708. Available: https://pubmed.ncbi.nlm.nih.gov/18985537/ 

[8] M. Reincke and M. Fleseriu, “Cushing Syndrome,” JAMA (Chicago, Ill.) , vol. 330, no. 2, p. 170, Jul. 2023, doi: 10.1001/jama.2023.11305. Available: https://pubmed.ncbi.nlm.nih.gov/37432427/ 

[9] “Congenital adrenal hyperplasia,” PubMed , Jan. 01, 2024. Available: https://pubmed.ncbi.nlm.nih.gov/28846271/ 

[10] “Adrenal adenoma,” PubMed , Jan. 01, 2024. Available: https://pubmed.ncbi.nlm.nih.gov/30969728/ 

[11] F. Wang et al. , “CT and MRI of adrenal gland pathologies,” Quantitative Imaging in Medicine and Surgery , vol. 8, no. 8, pp. 853–875, Sep. 2018, doi: 10.21037/qims.2018.09.13. Available: https://pubmed.ncbi.nlm.nih.gov/30306064/ 

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